CAJ | 학술논문

对不结球白菜细胞质雄性不育系S2108A和Y3611A及其相应的保持系S2108B和Y3611B进行了花器官形态比较和花药发育细胞学观察,结果表明:两套不育系和保持系之间在花器官的多个性状上差异极显著,而两个不育系S2108A和Y3611A之间除花蕾大小、雌蕊长和花瓣长有差异外,其他均不显著,说明从不同不育源转育来的两个不育系在花器官外形上差异不大;两个不育系花药败育表现均起始于四分体时期,且由于绒毡层的液泡化和径向膨大,挤压四分体小孢子导致败育,表明败育与绒毡层的不正常发育有关.此外,与Y3611A不同,在不育系S2108A中还存在另一种败育形式,绒毡层细胞壁发生融合,成为紧贴药室壁的类似于变形型绒毡层的周原质团,部分细胞质流人药室腔,包裹粘连四分体小孢子,成为染色很深的不连续团块状物,花药败育;两不育系花药发育存在花粉囊数目的变异,且花粉囊发育时期不同步,个别花粉囊较正常花粉囊发育推迟,在不育系Y3611A中还有巨型花粉囊的现象.
대불결구백채세포질웅성불육계S2108A화Y3611A급기상응적보지계S2108B화Y3611B진행료화기관형태비교화화약발육세포학관찰,결과표명:량투불육계화보지계지간재화기관적다개성상상차이겁현저,이량개불육계S2108A화Y3611A지간제화뢰대소、자예장화화판장유차이외,기타균불현저,설명종불동불육원전육래적량개불육계재화기관외형상차이불대;량개불육계화약패육표현균기시우사분체시기,차유우융전층적액포화화경향팽대,제압사분체소포자도치패육,표명패육여융전층적불정상발육유관.차외,여Y3611A불동,재불육계S2108A중환존재령일충패육형식,융전층세포벽발생융합,성위긴첩약실벽적유사우변형형융전층적주원질단,부분세포질류인약실강,포과점련사분체소포자,성위염색흔심적불련속단괴상물,화약패육;량불육계화약발육존재화분낭수목적변이,차화분낭발육시기불동보,개별화분낭교정상화분낭발육추지,재불육계Y3611A중환유거형화분낭적현상.
The morphologic comparison of flower organ and anatomical observations were conducted on male sterile lines S2108A and Y3611 A and their maintainer lines S2108B and Y3611B in Brassica rapa ssp. chinensis. The results showed that there were significant differences in several characters of blossom organ between two set of male sterile lines and their maintainer lines, while between the two male sterile lines S2108A and Y3611A,the difference was not found except in the length of flower bud pistil and length of petal, suggesting that no significant difference in blossom morphology between the two male sterile lines transferred from different origin. The anther a-bortion of the two male sterile lines started from tetrad period,that due to taptum's vacuole changing and radial ex-pandation,extrusion tetrad microspore and result in fail development,indicating the anther abortion had relationship with taptum abnormally development. Moreover, different from Y3611A, there is another anther abortion style in S2108A,taptum cell wall is fused,turn into periplasmodium like amoeboid tapetum cling to powder chamber wall, part of cytoplasm afflux into powder chamber, wrap and conglutinate tetrad microspore, the anther abortion. The number of clinandrium of the two male sterile lines is different,and development period of clinandrium is asynchro-ny,some clinandrium is late. In addition, huge clinandrium is found in the line of Y3611A.