CAJ | 학술논문

从作者所在实验室保存的3株白腐真菌中筛选到一株液态发酵产漆酶的密孔菌Pycnop-orus sp.SYBC-L1,以漆酶活力为指标,采用正交试验法,优化了Pycnoporus sp.SYBC-L1分泌漆酶的培养基:麸皮水煮液60 g/L,葡萄糖60 g/L,豆粕粉15 g/L,CuSO_4·5H_2O 1.0 mmol/L;培养条件:初始pH 3.0,装液量50 mL/250 mL,30℃、200 r/min培养13 d,漆酶活力达24.95 U/mL,为优化前的36.16倍.
종작자소재실험실보존적3주백부진균중사선도일주액태발효산칠매적밀공균Pycnop-orus sp.SYBC-L1,이칠매활력위지표,채용정교시험법,우화료Pycnoporus sp.SYBC-L1분비칠매적배양기:부피수자액60 g/L,포도당60 g/L,두박분15 g/L,CuSO_4·5H_2O 1.0 mmol/L;배양조건:초시pH 3.0,장액량50 mL/250 mL,30℃、200 r/min배양13 d,칠매활력체24.95 U/mL,위우화전적36.16배.
In this study, the nutritional and environmental conditions of a laccase produce strain, Pycnoporus sp. SYBC-L1, were investigated and optimiz ed though single factor experiments and orthogonal experiments. The optimum conditions described as follows: wheat bran cooking liquor 60 g/L, glucose 60 g/L, soybean powder 15 g/L and CuSO_·5H_2O 1.0 mmol/L, pH 3. 0, temperature 30 ℃, and culture volumn 50 mL/250 mL, respectively. With those optimum conditions, the laccase activity reached at 24. 95 U/mL after 13 days culture.