CAJ | 학술논문

从理化性能、离子交换容量、静态和动态吸附性能等方面对国产快流速CM-琼脂糖弱酸性阳离子交换介质进行了综合考察和评价,并应用该介质进行了鸡蛋清中溶茵酶的分离.结果发现,国产介质的理化性能符合要求,离子交换容量达到了187 mmol/mL,高于国外商品介质;对模型蛋白溶菌酶的静态和动态吸附容量分别达到了107.0 mg/mL和90.3 mg/mL(高流速下),同样略高于国外商品介质,显示能够满足蛋白质的有效吸附;离子交换层析分离蛋清溶菌酶较为成功,纯度在95%以上,产量达到了4.1 mg/mL鸡蛋清.
종이화성능、리자교환용량、정태화동태흡부성능등방면대국산쾌류속CM-경지당약산성양리자교환개질진행료종합고찰화평개,병응용해개질진행료계단청중용인매적분리.결과발현,국산개질적이화성능부합요구,리자교환용량체도료187 mmol/mL,고우국외상품개질;대모형단백용균매적정태화동태흡부용량분별체도료107.0 mg/mL화90.3 mg/mL(고류속하),동양략고우국외상품개질,현시능구만족단백질적유효흡부;리자교환층석분리단청용균매교위성공,순도재95%이상,산량체도료4.1 mg/mL계단청.
The national cation exchanger (Zhengguang CM-Agrose Fast Flow) was integrated evaluated on its physic properties, functional group density, static and dynamic adsorption properties. Besides, the adsorbents were used to purification of lysozyme from egg white. As a result, it was found that the physic properties of CM-Agrose FF include density, water content and size distribution are up to the requirement. The functional group density reaches 187 mmol/ mL, which is higher than CM Sepharose FF. The static and dynamic adsorption of lysozyme reach 107.0 and 90.3 mg/mL (100 cm/h), respectively, which indicated that the adsorbents could fulfil the efficient adsorption of proteins. Lysozyme was successfully purified from egg white using the cation exchanger. The purity of lysozyme was more than 95% and the yield reached 4. 1 mg/mL egg white.