农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2010年
4期
636-641
,共6页
杨野%郭再华%叶志娟%耿明建%王伟%宁大伟%赵竹青
楊野%郭再華%葉誌娟%耿明建%王偉%寧大偉%趙竹青
양야%곽재화%협지연%경명건%왕위%저대위%조죽청
小麦%铝毒%根际pH%H+-ATPase
小麥%鋁毒%根際pH%H+-ATPase
소맥%려독%근제pH%H+-ATPase
wheat%aluminum toxicity%rhizosphere pH%H+-ATPase
Al3+是植物铝毒害的主要形态,而其活性受环境pH值的影响,H+-ATPase通过调节根的质子分泌改变根际pH值.为探讨铝胁迫下根际pH值变化与小麦耐铝性的关系,以小麦品种ET8(耐铝型)、ES8(铝敏感型)为试验材料,采用溶液培养的方法对铝胁迫下根际pH值及根尖H*-ATPase活性变化进行了研究.结果表明,铝处理条件下,小麦根际pH值随培养时间的延长而升高;随培养液中铝浓度的增加,根际pH值上升幅度下降,相同铝浓度处理条件下ET8根际pH值显著高于ES8.根际pH值与根尖铝含量呈极显著负相关(R2=0.932 1),与根相对伸长率呈极显著正相关(R2=0.858 5),表明小麦通过提高根际pH值降低根尖铝含量,减轻铝毒害.根尖H+-ATPase活性随铝处理浓度升高而显著降低,100 μmol·L-1 Al处理24 h ET8和ES8根尖H+-ATPase活性分别为各自无铝处理的69.8%和60.0%,根尖H+-ATPase相对活性与根际pH值呈极显著负相关(R2=0.831 9).温度显著影响根的伸长,低温处理(9℃)根际pH值显著高于常温处理(25℃),而根尖铝含量却显著低于常温处理.表明小麦通过根尖H+-ATPase提高根际pH值降低铝毒害.综上所述,铝胁迫下小麦可通过提高根际pH值减轻铝毒害,不同耐铝性小麦品种根际pH值的显著差异是耐铝性差异显著的原因之一.
Al3+是植物鋁毒害的主要形態,而其活性受環境pH值的影響,H+-ATPase通過調節根的質子分泌改變根際pH值.為探討鋁脅迫下根際pH值變化與小麥耐鋁性的關繫,以小麥品種ET8(耐鋁型)、ES8(鋁敏感型)為試驗材料,採用溶液培養的方法對鋁脅迫下根際pH值及根尖H*-ATPase活性變化進行瞭研究.結果錶明,鋁處理條件下,小麥根際pH值隨培養時間的延長而升高;隨培養液中鋁濃度的增加,根際pH值上升幅度下降,相同鋁濃度處理條件下ET8根際pH值顯著高于ES8.根際pH值與根尖鋁含量呈極顯著負相關(R2=0.932 1),與根相對伸長率呈極顯著正相關(R2=0.858 5),錶明小麥通過提高根際pH值降低根尖鋁含量,減輕鋁毒害.根尖H+-ATPase活性隨鋁處理濃度升高而顯著降低,100 μmol·L-1 Al處理24 h ET8和ES8根尖H+-ATPase活性分彆為各自無鋁處理的69.8%和60.0%,根尖H+-ATPase相對活性與根際pH值呈極顯著負相關(R2=0.831 9).溫度顯著影響根的伸長,低溫處理(9℃)根際pH值顯著高于常溫處理(25℃),而根尖鋁含量卻顯著低于常溫處理.錶明小麥通過根尖H+-ATPase提高根際pH值降低鋁毒害.綜上所述,鋁脅迫下小麥可通過提高根際pH值減輕鋁毒害,不同耐鋁性小麥品種根際pH值的顯著差異是耐鋁性差異顯著的原因之一.
Al3+시식물려독해적주요형태,이기활성수배경pH치적영향,H+-ATPase통과조절근적질자분비개변근제pH치.위탐토려협박하근제pH치변화여소맥내려성적관계,이소맥품충ET8(내려형)、ES8(려민감형)위시험재료,채용용액배양적방법대려협박하근제pH치급근첨H*-ATPase활성변화진행료연구.결과표명,려처리조건하,소맥근제pH치수배양시간적연장이승고;수배양액중려농도적증가,근제pH치상승폭도하강,상동려농도처리조건하ET8근제pH치현저고우ES8.근제pH치여근첨려함량정겁현저부상관(R2=0.932 1),여근상대신장솔정겁현저정상관(R2=0.858 5),표명소맥통과제고근제pH치강저근첨려함량,감경려독해.근첨H+-ATPase활성수려처리농도승고이현저강저,100 μmol·L-1 Al처리24 h ET8화ES8근첨H+-ATPase활성분별위각자무려처리적69.8%화60.0%,근첨H+-ATPase상대활성여근제pH치정겁현저부상관(R2=0.831 9).온도현저영향근적신장,저온처리(9℃)근제pH치현저고우상온처리(25℃),이근첨려함량각현저저우상온처리.표명소맥통과근첨H+-ATPase제고근제pH치강저려독해.종상소술,려협박하소맥가통과제고근제pH치감경려독해,불동내려성소맥품충근제pH치적현저차이시내려성차이현저적원인지일.
Al3+is regarded as the greatest stress to plant and its activity is affected by environmental pH.Rhizosphere pH can be changed by H+-ATPase which regulates the secretion of proton.To explain the relationship between changes of rhizosphere pH and Al-resistant of wheat (Triticum aestivum L.),the changes of rhizosphere pH and H+-ATPase activity of root apices of ET8(Al-resistant)and ES8(Al-sensitive)were investigated.The results were as follows:the rhizosphere pH rose with the prolonging of Al treatments,and the rising amplitude of pH decreased with the increasing of Al concentrations.The rhizosphere pH of ET8 was much higher than that of ES8 under the same Al treatments.Significant correlations were obtained between rhizosphere pH and the relative root elongation(R2=0.858 5)or Al content in root apices(R2=0.932 1).The increase of the rhizosphere pH led to the alleviation of Al-toxieity and the decrease of Al content in root apices.The H+-ATPase activities decreased with the increase of Al concentrations.There were only 69.8% and 60.0% of control treatments of ET8 and ES8 respectively after treatments of 100 μmol·L-1 Al for 24 h.A good correlation between the relative H+-ATPase activities and the rhizosphere pH was obtained(R2=0.831 9).In addition,root growth was seriously affected by low temperature(9℃).The rhizosphere pH at low temperature(9℃)was significantly higher and the root apices Al content was significantly lower than that of normal temperature(25℃)treatments.Collectively,these results suggest that Al toxicity can be alleviated by the increase of rhizosphere pH in relation to H+-ATPase.The significant difference in rhizosphere pH is one of main reasons that cause the Al-resistant difference among different Al-tolerant wheat species.
