农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
4期
72-74
,共3页
泥火山%宏基因组DNA%新疆
泥火山%宏基因組DNA%新疆
니화산%굉기인조DNA%신강
Mud volcano%Metagenomic DNA%Xinjiang
[目的]寻找一种有效的泥火山土壤宏基因组DNA的提取方法.[方法]分别采用CTAB法、SDS加酶法、实验室改进法、试剂盒法提取泥火山土壤宏基因组,比较4种方法的提取效果.[结果]试剂盒法的提取效率最高、纯度最好;实验室改进法次之;SDS加酶法提取量最多.实验室改进法获得的DNA,稀释10倍可直接用于PCR扩增.[结论]从经济和DNA质量的角度考虑,实验室改进法可作为新疆泥火山土壤宏基因组DNA提取最有效的方法.
[目的]尋找一種有效的泥火山土壤宏基因組DNA的提取方法.[方法]分彆採用CTAB法、SDS加酶法、實驗室改進法、試劑盒法提取泥火山土壤宏基因組,比較4種方法的提取效果.[結果]試劑盒法的提取效率最高、純度最好;實驗室改進法次之;SDS加酶法提取量最多.實驗室改進法穫得的DNA,稀釋10倍可直接用于PCR擴增.[結論]從經濟和DNA質量的角度攷慮,實驗室改進法可作為新疆泥火山土壤宏基因組DNA提取最有效的方法.
[목적]심조일충유효적니화산토양굉기인조DNA적제취방법.[방법]분별채용CTAB법、SDS가매법、실험실개진법、시제합법제취니화산토양굉기인조,비교4충방법적제취효과.[결과]시제합법적제취효솔최고、순도최호;실험실개진법차지;SDS가매법제취량최다.실험실개진법획득적DNA,희석10배가직접용우PCR확증.[결론]종경제화DNA질량적각도고필,실험실개진법가작위신강니화산토양굉기인조DNA제취최유효적방법.
[Objective] To seek one effective extraction method of metagenomic DNA from mud volcano. [Method] The metagenomic DNA from mud volcano was extracted by CTAB extraction method, SDS-enzyme method, improved method, reagent kit method. The extraction of four kinds of methods were compared. [Result] The extracted rate in reagent sets method was the highest, next was improved method, the extracted quantity in SDS-enzyme method was maximum. DNA extracted by the improved method was diluted ten times for PCR. [Conclusion] Considering economy and purity, the improved method can be used as one effective extraction method of metagenomic DNA from mud volcano.
