农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2011年
3期
389-393
,共5页
邢瑞%车轶%崔勇华%徐世清
邢瑞%車軼%崔勇華%徐世清
형서%차질%최용화%서세청
微波%认知功能%NMDA受体%NR1亚基%NR2亚基
微波%認知功能%NMDA受體%NR1亞基%NR2亞基
미파%인지공능%NMDA수체%NR1아기%NR2아기
Microwave%Cognitive function%NMDA receptor%NR1 subunit%NR2 subunit
[目的]研究微波对鸡胚生长发育及雏鸡认知功能的影响.[方法]采用磁控管发射2450 MHz的微波来模拟微波辐射源辐射种蛋,直到雏鸡孵出后,分别采用一次性被动回避学习和RT-PCR检测微波对雏鸡的认知功能和NMDA受体NRI亚基与NR2亚基表达量的影响.[结果]微波辐射后暴露组的回避率显著低于对照组,特别在辐射强度最高组的回避率极显著低于对照组.同时暴露组有2组雏鸡体重增加,其中一组的孵化时间有所增加.经RT-PCR分析,NR2亚基表达量在第10天而及15天时都上调,NR1亚基表达量只在第15天时才下调.[结论]微波对个体发育具有一定影响,通过使端脑中NMDA受体结构组成和功能发生改变,使自身的调节能力下降,从而对认知功能有一定损害.
[目的]研究微波對鷄胚生長髮育及雛鷄認知功能的影響.[方法]採用磁控管髮射2450 MHz的微波來模擬微波輻射源輻射種蛋,直到雛鷄孵齣後,分彆採用一次性被動迴避學習和RT-PCR檢測微波對雛鷄的認知功能和NMDA受體NRI亞基與NR2亞基錶達量的影響.[結果]微波輻射後暴露組的迴避率顯著低于對照組,特彆在輻射彊度最高組的迴避率極顯著低于對照組.同時暴露組有2組雛鷄體重增加,其中一組的孵化時間有所增加.經RT-PCR分析,NR2亞基錶達量在第10天而及15天時都上調,NR1亞基錶達量隻在第15天時纔下調.[結論]微波對箇體髮育具有一定影響,通過使耑腦中NMDA受體結構組成和功能髮生改變,使自身的調節能力下降,從而對認知功能有一定損害.
[목적]연구미파대계배생장발육급추계인지공능적영향.[방법]채용자공관발사2450 MHz적미파래모의미파복사원복사충단,직도추계부출후,분별채용일차성피동회피학습화RT-PCR검측미파대추계적인지공능화NMDA수체NRI아기여NR2아기표체량적영향.[결과]미파복사후폭로조적회피솔현저저우대조조,특별재복사강도최고조적회피솔겁현저저우대조조.동시폭로조유2조추계체중증가,기중일조적부화시간유소증가.경RT-PCR분석,NR2아기표체량재제10천이급15천시도상조,NR1아기표체량지재제15천시재하조.[결론]미파대개체발육구유일정영향,통과사단뇌중NMDA수체결구조성화공능발생개변,사자신적조절능력하강,종이대인지공능유일정손해.
[Objective] The research aimed to study the effects of microwave on the chick embryo development and the cognitive function of chickling. [Method] The microwave which was transmitted by the permatron and was 2450 MHz was used to simulate the microwave radiation source to radiate the hatching eggs until the chickling was hatched out. The disposable passive avoidance learning and RT-PCR were respectively used to detect the influences of microwave on the cognitive function of chickling and the expression amounts of NMDA receptor NR1 and NR2subunits. [Result] After the microwave radiation, the avoidance rate of exposed group was significantly lower than that in the control group. Especially the avoidance rate of highest radiation intensity group was extremely significantly lower than that in the control group. Meanwhile, the body weights of two groups of chickling in the exposed group increased, and the hatching time in one group increased. Via RT-PCR analysis,the expression amount of NR2 subunit increased on the 10th day and the 15th day. The expression amount of NR1 subunit only decreased on the 15th day. [Conclusion] The microwave had the certain influence on the individual development. By changing the structure composition and function of NMDA receptor in the endbrain, the microwave made the self-regulation ability of chickling decline, which had the certain damage on the cognitive function.