国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2009年
11期
658-661
,共4页
慢性阻塞性肺疾病%颗粒溶素%γ干扰素%白介素2
慢性阻塞性肺疾病%顆粒溶素%γ榦擾素%白介素2
만성조새성폐질병%과립용소%γ간우소%백개소2
Chronic obstructive pulmonary disease%Granulysin%Interferon-γ%Interleukin-2
目的 探讨慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者不同病期颗粒溶素表达特点以及和γ干扰素(interferon-γ,IFN-γ)、白介素2(interleukin-2,IL-2)之间的关系.方法 采用免疫细胞化学技术检测108例COPD患者及46名健康对照组外周血淋巴细胞的表达水平,采用双抗体夹心ABC-ELISA法同步测定血清IFN-γ、IL-2浓度.结果 颗粒溶素在COPD患者和健康对照组外周血淋巴细胞中的阳性表达率比较差异有统计学意义(P<0.01).颗粒溶素在健康对照组、临床缓解期、急性发作期的外周血淋巴细胞中表达比较差异有统计学意义(P<0.01).COPD患者急性发作期血清IFN-γ、IL-2浓度较临床缓解期和健康对照组显著增高(P<0.01).COPD患者急性发作期和临床缓解期颗粒溶素表达与血清IFN-γ、IL-2浓度变化均呈正相关,并随着病情变化存在明显消长关系.结论 颗粒溶素参与了COPD炎症过程,起着清除病原体的积极作用.IFN-γ、IL-2与颗粒溶素在消除病原体方面可能起着相互协同的作用,可能影响颗粒溶素的释放.
目的 探討慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者不同病期顆粒溶素錶達特點以及和γ榦擾素(interferon-γ,IFN-γ)、白介素2(interleukin-2,IL-2)之間的關繫.方法 採用免疫細胞化學技術檢測108例COPD患者及46名健康對照組外週血淋巴細胞的錶達水平,採用雙抗體夾心ABC-ELISA法同步測定血清IFN-γ、IL-2濃度.結果 顆粒溶素在COPD患者和健康對照組外週血淋巴細胞中的暘性錶達率比較差異有統計學意義(P<0.01).顆粒溶素在健康對照組、臨床緩解期、急性髮作期的外週血淋巴細胞中錶達比較差異有統計學意義(P<0.01).COPD患者急性髮作期血清IFN-γ、IL-2濃度較臨床緩解期和健康對照組顯著增高(P<0.01).COPD患者急性髮作期和臨床緩解期顆粒溶素錶達與血清IFN-γ、IL-2濃度變化均呈正相關,併隨著病情變化存在明顯消長關繫.結論 顆粒溶素參與瞭COPD炎癥過程,起著清除病原體的積極作用.IFN-γ、IL-2與顆粒溶素在消除病原體方麵可能起著相互協同的作用,可能影響顆粒溶素的釋放.
목적 탐토만성조새성폐질병(chronic obstructive pulmonary disease,COPD)환자불동병기과립용소표체특점이급화γ간우소(interferon-γ,IFN-γ)、백개소2(interleukin-2,IL-2)지간적관계.방법 채용면역세포화학기술검측108례COPD환자급46명건강대조조외주혈림파세포적표체수평,채용쌍항체협심ABC-ELISA법동보측정혈청IFN-γ、IL-2농도.결과 과립용소재COPD환자화건강대조조외주혈림파세포중적양성표체솔비교차이유통계학의의(P<0.01).과립용소재건강대조조、림상완해기、급성발작기적외주혈림파세포중표체비교차이유통계학의의(P<0.01).COPD환자급성발작기혈청IFN-γ、IL-2농도교림상완해기화건강대조조현저증고(P<0.01).COPD환자급성발작기화림상완해기과립용소표체여혈청IFN-γ、IL-2농도변화균정정상관,병수착병정변화존재명현소장관계.결론 과립용소삼여료COPD염증과정,기착청제병원체적적겁작용.IFN-γ、IL-2여과립용소재소제병원체방면가능기착상호협동적작용,가능영향과립용소적석방.
Objective To detect the expression of granulysin in peripheral blood lymphocytes and its correlation analysis with interferon-γ (IFN-γ), interleukin-2 (IL-2) serum concentrations in the immunopathological process of chronic obstructive pulmonary disease(COPD). Methods The expression of granulysin in peripheral blood lymphocytes from 108 patients with COPD and 46 healthy controls was evaluated by means of immunocytochemical analysis. Meantime, the concentrations of IFN-γ and IL-2 in serum from these patients and healthy controls were also measured by means of double antibody sandwich ABC-ELISA. Results Compared with control subjects,patients with COPD showed an increased expression of granulysin(P <0.01). During stables,compared with exacerbations, the expression of granulysin was reduced(P <0.01), but compared with controls, it was still higher(P <0.01). The serum concentrations of IFN-γ,IL-2 in acute exacerbations were significant higher than those in stables and controls(P <0.01).There were positive correlation between the expression of granulysin in acute exacerbations and stables and the change of IFN-γ, IL-2 serum concentration. Conclusions Granulysin may involve in the process of inflammation in COPD by means of eliminating pathogens. There was positive correlation between the expression of granulysin and IFN-γ, IL-2 serum concentration. IFN-γ and IL-2 may play synergetic roles in the process of eliminating pathogen and play a positive role in the release of granulysin.
