中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2012年
6期
550-555
,共6页
顾卫东%于布为%张富军%薛庆生%马政文%陆佩华
顧衛東%于佈為%張富軍%薛慶生%馬政文%陸珮華
고위동%우포위%장부군%설경생%마정문%륙패화
骨髓%干细胞移植%脊髓损伤%突起生长
骨髓%榦細胞移植%脊髓損傷%突起生長
골수%간세포이식%척수손상%돌기생장
Bone marrow%Stem cell transplantation%Spinal cord injury%Axonal growth
目的 研究骨髓间充质干细胞(BMSCs)移植对损伤脊髓神经功能恢复的影响及其机制.方法 采用纽约大学脊髓重物坠落仪制作大鼠脊髓损伤模型.脊髓损伤后7d,在损伤中心周围移植BMSCs( BMSCs组)或注射PBS( PBS组).采用BBB评分评价大鼠脊髓功能.测定脊髓空洞体积,透射电镜观察脊髓损伤中心轴突的数量.采用绿色荧光蛋白(GFP)转基因大鼠的BMSCs示踪移植细胞,观察BMSCs移植后在脊髓中的存活及向神经细胞分化的情况.采用脊髓埋管模型,研究BMSCs移植对损伤脊髓轴突再生的影响.结果 BMSCs组BBB评分显著高于PBS组,而其脊髓空洞体积明显小于PBS组.电镜下可见,在脊髓损伤中心BMSCs组的轴突数量明显多于PBS组.细胞移植后4周,在脊髓损伤中心周围可见大量BMSCs - GFP细胞.免疫荧光染色结果表明,脊髓移植的BMSCs并不表达神经元、星形胶质细胞和少突胶质细胞的表面标志性蛋白.脊髓埋管实验发现,在种有BMSCs的导管内可见有NF阳性的神经纤维的长入,而未种有BMSCs组的导管内未见神经纤维的生长.结论 脊髓损伤后进行BMSCs移植可促进损伤脊髓功能的恢复,其机制与BMSCs移植促进神经元轴突再生的作用有关.
目的 研究骨髓間充質榦細胞(BMSCs)移植對損傷脊髓神經功能恢複的影響及其機製.方法 採用紐約大學脊髓重物墜落儀製作大鼠脊髓損傷模型.脊髓損傷後7d,在損傷中心週圍移植BMSCs( BMSCs組)或註射PBS( PBS組).採用BBB評分評價大鼠脊髓功能.測定脊髓空洞體積,透射電鏡觀察脊髓損傷中心軸突的數量.採用綠色熒光蛋白(GFP)轉基因大鼠的BMSCs示蹤移植細胞,觀察BMSCs移植後在脊髓中的存活及嚮神經細胞分化的情況.採用脊髓埋管模型,研究BMSCs移植對損傷脊髓軸突再生的影響.結果 BMSCs組BBB評分顯著高于PBS組,而其脊髓空洞體積明顯小于PBS組.電鏡下可見,在脊髓損傷中心BMSCs組的軸突數量明顯多于PBS組.細胞移植後4週,在脊髓損傷中心週圍可見大量BMSCs - GFP細胞.免疫熒光染色結果錶明,脊髓移植的BMSCs併不錶達神經元、星形膠質細胞和少突膠質細胞的錶麵標誌性蛋白.脊髓埋管實驗髮現,在種有BMSCs的導管內可見有NF暘性的神經纖維的長入,而未種有BMSCs組的導管內未見神經纖維的生長.結論 脊髓損傷後進行BMSCs移植可促進損傷脊髓功能的恢複,其機製與BMSCs移植促進神經元軸突再生的作用有關.
목적 연구골수간충질간세포(BMSCs)이식대손상척수신경공능회복적영향급기궤제.방법 채용뉴약대학척수중물추락의제작대서척수손상모형.척수손상후7d,재손상중심주위이식BMSCs( BMSCs조)혹주사PBS( PBS조).채용BBB평분평개대서척수공능.측정척수공동체적,투사전경관찰척수손상중심축돌적수량.채용록색형광단백(GFP)전기인대서적BMSCs시종이식세포,관찰BMSCs이식후재척수중적존활급향신경세포분화적정황.채용척수매관모형,연구BMSCs이식대손상척수축돌재생적영향.결과 BMSCs조BBB평분현저고우PBS조,이기척수공동체적명현소우PBS조.전경하가견,재척수손상중심BMSCs조적축돌수량명현다우PBS조.세포이식후4주,재척수손상중심주위가견대량BMSCs - GFP세포.면역형광염색결과표명,척수이식적BMSCs병불표체신경원、성형효질세포화소돌효질세포적표면표지성단백.척수매관실험발현,재충유BMSCs적도관내가견유NF양성적신경섬유적장입,이미충유BMSCs조적도관내미견신경섬유적생장.결론 척수손상후진행BMSCs이식가촉진손상척수공능적회복,기궤제여BMSCs이식촉진신경원축돌재생적작용유관.
Objective To investigate the effects of bone mesenchymal stem cells (BMSCs)transplantation on the neurological function recovery of injured spinal cord and the underlying mechanism.Methods Rats were subjected to contusive spinal cord injury by using NYU spinal cord contusive impactor system ( NYC lmpactor).Seven days after spinal cord injury,the transplantation of BMSCs ( BMSCs group) or injection of PBS ( PBS group) was performed around the epicenter of injured spinal cord in rats.Basso-Beattie-Bresnahan (BBB) score was used to evaluate the function of spinal cord.The cavity volume of the injured spinal cord was measured and the axons in the injury center of spinal cord were examined under transmission electron microscopy.The BMSCs of the green fluorescent protein (GFP)transgenic rats were used to trace the transplanted cells and the survivor of BMSCs in the injured spinal cord and their differentiation into neural cells were observed.A mini-channel implantation model was employed to further investigate the role of BMSCs transplantation on the axonal regeneration.Results The BMSCs group showed a higher BBB score and a smaller lesion volume as compared with the PBS group.Transmission electron microscopy examination displayed that the number of axons in the BMSCs group was far more than that in the PBS group.A great number of BMSCs-GFP were founded around the center of the injured spinal cord at 4 weeks after BMSCs transplantation.lmmunohistochemistry showed that the implanted BMSCs-GFP did not express the surface marker of neurons,astrocytes and oligodendrocytes.In the mini-channel implantation model,NF-positive nerve fibers grew into the BMSCs-seeded channel,while there were no nerve fibers in the channel without seeding of BMSCs.Conclusions The BMSCs transplantation for the injured spinal cord promotes its functional recovery,and the related mechanism is in correlation with BMSCs transplantation inducing axonal regeneration.
