中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2011年
2期
93-97
,共5页
2,4,6-三硝基苯磺酸%结肠炎%黄芪多糖%树突状细胞%炎症性肠病
2,4,6-三硝基苯磺痠%結腸炎%黃芪多糖%樹突狀細胞%炎癥性腸病
2,4,6-삼초기분광산%결장염%황기다당%수돌상세포%염증성장병
2,4,6-trinitrobenzenesulfonic acid%Colitis%Astragalus polysaccharides%Dendritic cells%Inflammatory bowel disease
目的 观察黄芪多糖(APS)对2,4,6-三硝基苯磺酸(TNBS)诱导的实验性结肠炎大鼠肠道炎症及树突状细胞(DCs)的作用.方法 44只雄性SD大鼠用简单随机抽样法分为4组(n=11):空白对照组、TNBS模型组、APS治疗组、5-氨基水杨酸(5-ASA)治疗组.除空白对照组外,其余3组均给予TNBS灌肠造模.造模后第2天,APS治疗组和5-ASA治疗组分别给予APS(0.75 g·kg-1·d-1)和5-ASA(100 mg·kg-1·d-1)灌胃治疗10 d.治疗结束后处死大鼠取材.对大鼠结肠炎症进行评估,包括疾病活动指数(DAI)评分、大体形态损伤评分、病理组织学评分和髓过氧化物酶(MPO)活性测定;流式细胞仪检测DCs表面标志MHCⅡ和CD86的表达.结果 APS治疗组较TNBS组大鼠的DAI评分(P=0.007)、大体形态损伤评分(P=0.017)、病理组织学评分(P=0.016)均显著降低,MPO活性亦下降,但差异无统计学意义(P=0.183).TNBS模型组大鼠肠系膜淋巴结DCs表面标志MHC Ⅱ和CD86的表达率较空白对照组(P=0.005,P=0.008)、APS治疗组(P=0.023,P=0.018)及5-ASA治疗组(P=0.017,P=0.013)均显著升高.结论 APS治疗可部分缓解TNBS诱导的大鼠实验性结肠炎,下调肠系膜淋巴结中活化的DCs.
目的 觀察黃芪多糖(APS)對2,4,6-三硝基苯磺痠(TNBS)誘導的實驗性結腸炎大鼠腸道炎癥及樹突狀細胞(DCs)的作用.方法 44隻雄性SD大鼠用簡單隨機抽樣法分為4組(n=11):空白對照組、TNBS模型組、APS治療組、5-氨基水楊痠(5-ASA)治療組.除空白對照組外,其餘3組均給予TNBS灌腸造模.造模後第2天,APS治療組和5-ASA治療組分彆給予APS(0.75 g·kg-1·d-1)和5-ASA(100 mg·kg-1·d-1)灌胃治療10 d.治療結束後處死大鼠取材.對大鼠結腸炎癥進行評估,包括疾病活動指數(DAI)評分、大體形態損傷評分、病理組織學評分和髓過氧化物酶(MPO)活性測定;流式細胞儀檢測DCs錶麵標誌MHCⅡ和CD86的錶達.結果 APS治療組較TNBS組大鼠的DAI評分(P=0.007)、大體形態損傷評分(P=0.017)、病理組織學評分(P=0.016)均顯著降低,MPO活性亦下降,但差異無統計學意義(P=0.183).TNBS模型組大鼠腸繫膜淋巴結DCs錶麵標誌MHC Ⅱ和CD86的錶達率較空白對照組(P=0.005,P=0.008)、APS治療組(P=0.023,P=0.018)及5-ASA治療組(P=0.017,P=0.013)均顯著升高.結論 APS治療可部分緩解TNBS誘導的大鼠實驗性結腸炎,下調腸繫膜淋巴結中活化的DCs.
목적 관찰황기다당(APS)대2,4,6-삼초기분광산(TNBS)유도적실험성결장염대서장도염증급수돌상세포(DCs)적작용.방법 44지웅성SD대서용간단수궤추양법분위4조(n=11):공백대조조、TNBS모형조、APS치료조、5-안기수양산(5-ASA)치료조.제공백대조조외,기여3조균급여TNBS관장조모.조모후제2천,APS치료조화5-ASA치료조분별급여APS(0.75 g·kg-1·d-1)화5-ASA(100 mg·kg-1·d-1)관위치료10 d.치료결속후처사대서취재.대대서결장염증진행평고,포괄질병활동지수(DAI)평분、대체형태손상평분、병리조직학평분화수과양화물매(MPO)활성측정;류식세포의검측DCs표면표지MHCⅡ화CD86적표체.결과 APS치료조교TNBS조대서적DAI평분(P=0.007)、대체형태손상평분(P=0.017)、병리조직학평분(P=0.016)균현저강저,MPO활성역하강,단차이무통계학의의(P=0.183).TNBS모형조대서장계막림파결DCs표면표지MHC Ⅱ화CD86적표체솔교공백대조조(P=0.005,P=0.008)、APS치료조(P=0.023,P=0.018)급5-ASA치료조(P=0.017,P=0.013)균현저승고.결론 APS치료가부분완해TNBS유도적대서실험성결장염,하조장계막림파결중활화적DCs.
Objective To investigate the effect of Astragalus polysaccharides (APS) on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats and on dendritic cells (DCs) in mesenteric lymph nodes.Methods Forty-four male Sprague-Dawley rats were randomly divided into four groups (n = 11) using simple random sampling: normal control group, TNBS group, APS group, and 5-aminosalicylic acid (5-ASA) group.Experimental colitis was induced in rats by TNBS enema in the last three groups.Rats in APS and 5-ASA groups were treated by gavage with APS (0.75 g ? kg-1 ? d-1) and 5-ASA (100 mg ? kg-1 ? d-1) on the 10 consecutive days following TNBS administration.The rats were then sacrificed and the colonic inflammatory scores of rats were measured, including the scores of disease activity index ( DAI) , macroscopic lesions, and histological damages,as well as the activity of myeloperoxidase (MPO).The expressions of major histocompatibility complex class Ⅱ(MHC Ⅱ ) and costimulatory molecule CD86 on DCs were determined by flow cytometry.Results Compared with the TNBS group, APS group had significantly decreased scores of DAI ( P = 0.007 ) , macroscopic lesions (P =0.017), and histological damages (P = 0.016).Moreover, its level of the activity of MPO dropped but without statistical significance (P =0.183).TNBS group had significantly higher expressions of MHC Ⅱand CD86 molecules on DCs than the normal control group (P = 0.005, P = 0.008), APS group (P = 0.023, P = 0.018), and 5-ASA group (P = 0.017, P=0.013).Conclusion APS may attenuate TNBS-induced colitis in rats and downregulate the activation of DCs in mesenteric lymph nodes.
