CAJ | 학술논문

目的观察自组装peptide胶对神经干细胞(NSCs)在大鼠急性期损伤脊髓中的细胞分化的影响.方法分离、培养和鉴定大鼠NSCs与自组装peptide胶共培养;SD大鼠25只采用NYU-Ⅱ型脊髓打击器制作T10脊髓损伤模型;急性期于损伤脊髓区分别行注射移植,其中联合细胞移植组(n=10)、单纯细胞移植组(n=10)及对照组(n=5).术后第2、4、8周取损伤部位脊髓,免疫组织化学染色检测移植细胞的分化.结果成功建立大鼠NSCs的体外培养体系;移植的NSCs在大鼠脊髓内存活超过8周,单纯移植组、细胞分化比例较低,分化的NSCs主要为表达胶质纤维酸性蛋白(GFAP)抗原细胞,未见NSCs分化为表达微管相关蛋白(MAP2)标志抗原细胞;联合移植组,细胞分化比例较高;NSCs可分化为表达MAP2、Oligo、GFAP标志抗原细胞.结论自组装peptide胶能够提高神经干细胞在大鼠急性期损伤脊髓中的分化比率,并有部分细胞可分化为表达神经元特异抗原的细胞.
목적 관찰자조장peptide효대신경간세포(NSCs)재대서급성기손상척수중적세포분화적영향.방법 분리、배양화감정대서NSCs여자조장peptide효공배양;SD대서25지채용NYU-Ⅱ형척수타격기제작T10척수손상모형;급성기우손상척수구분별행주사이식,기중연합세포이식조(n=10)、단순세포이식조(n=10)급대조조(n=5).술후제2、4、8주취손상부위척수,면역조직화학염색검측이식세포적분화.결과 성공건립대서NSCs적체외배양체계;이식적NSCs재대서척수내존활초과8주,단순이식조、세포분화비례교저,분화적NSCs주요위표체효질섬유산성단백(GFAP)항원세포,미견NSCs분화위표체미관상관단백(MAP2)표지항원세포;연합이식조,세포분화비례교고;NSCs가분화위표체MAP2、Oligo、GFAP표지항원세포.결론 자조장peptide효능구제고신경간세포재대서급성기손상척수중적분화비솔,병유부분세포가분화위표체신경원특이항원적세포.
Objective To study the influence of self-assembled peptide nanofiber scaffold on the proliferation of the neural stem cells (NSCs) transplanted in rats' injured spinal cord in the acute phase. Methods NSCs in rats were isolated,cultured and identified,and co-cultured with self-assembled peptide nanofiber scaffold. Twenty-five SD rats were hit with NYU-Ⅱ strike machine at T10 to establish rats' spinal-cord injury model. Transplant cells and peptide with injection in acute phase of injured spinal cords,in which the united cell transplantation group (n = 10) ,a simple cell transplantation group (n = 10) and the control group (n =5).2,4,8 weeks after the transplantation,immunohistechemistry was made to test the cells' divi-sion. Results The rats' NSCs culture system was successfully established in vitro. NSCs transplanted in the injured spinal cords could live more than 8 weeks. In simply transplantation group,NSCs had a lower propor-tion of cell differentiation,and no microtubule-associated protein 2 (MAP2) marked cells differentiation hap-pened ,and a little glial fibrillary acidic protein (GFAP) marked cells differentiation. In united transplantation group, NSCs had a higher proportion of cells differentiation, and could differentiate into MAP2/Oligo/GFAP marked cells. Conclusion Self-assembled peptide nanofiber scaffold could improve the neural stem cells' differentiation proportion in the acute phase of rats' injured spinal cord,and some of the cells could differenti-ate into neuron-specific antigen cells.