中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
2期
304-306,封3
,共4页
卢冰%刘红山%胡伟明%田伯乐%张肇达
盧冰%劉紅山%鬍偉明%田伯樂%張肇達
로빙%류홍산%호위명%전백악%장조체
再灌注损伤%胰腺%模型,动物
再灌註損傷%胰腺%模型,動物
재관주손상%이선%모형,동물
Reperfusion injury%Pancreas%Model,animal
目的 建立一种简便实用的大鼠经腹主动脉胰腺缺血再灌注模型.方法 将60只Wistar大鼠随机分为:新模型组、对照组和假手术组,每组20只.采用显微外科技术游离胰腺,胰腺灌注后阻断血流2 h,恢复再灌注后0、2、4、6 h,切取胰腺体尾部组织,检测湿/干重比、行组织病理学分析及血清淀粉酶和脂肪酶检测.结果 再灌注后,新模型组血清淀粉酶2 h(3127.80±150.85)U/L、4 h(3122.80±131.52)U/L、6 h(2585.20±161.06)U/L;血清脂肪酶2 h(446.00±181.54)U/L、4 h(517.40±165.22)U/L、6 h(475.20±170.37)U/L;均随再灌注时间延长有明显升高趋势(P<0.05),胰腺组织病理切片呈现逐渐加重的炎细胞浸润及组织水肿(P<0.05)等改变.结论 本模型手术操作简便、又能够较好地模拟临床胰腺缺血再灌注的过程,是研究胰腺缺血再灌注损伤的较理想动物模型.
目的 建立一種簡便實用的大鼠經腹主動脈胰腺缺血再灌註模型.方法 將60隻Wistar大鼠隨機分為:新模型組、對照組和假手術組,每組20隻.採用顯微外科技術遊離胰腺,胰腺灌註後阻斷血流2 h,恢複再灌註後0、2、4、6 h,切取胰腺體尾部組織,檢測濕/榦重比、行組織病理學分析及血清澱粉酶和脂肪酶檢測.結果 再灌註後,新模型組血清澱粉酶2 h(3127.80±150.85)U/L、4 h(3122.80±131.52)U/L、6 h(2585.20±161.06)U/L;血清脂肪酶2 h(446.00±181.54)U/L、4 h(517.40±165.22)U/L、6 h(475.20±170.37)U/L;均隨再灌註時間延長有明顯升高趨勢(P<0.05),胰腺組織病理切片呈現逐漸加重的炎細胞浸潤及組織水腫(P<0.05)等改變.結論 本模型手術操作簡便、又能夠較好地模擬臨床胰腺缺血再灌註的過程,是研究胰腺缺血再灌註損傷的較理想動物模型.
목적 건립일충간편실용적대서경복주동맥이선결혈재관주모형.방법 장60지Wistar대서수궤분위:신모형조、대조조화가수술조,매조20지.채용현미외과기술유리이선,이선관주후조단혈류2 h,회복재관주후0、2、4、6 h,절취이선체미부조직,검측습/간중비、행조직병이학분석급혈청정분매화지방매검측.결과 재관주후,신모형조혈청정분매2 h(3127.80±150.85)U/L、4 h(3122.80±131.52)U/L、6 h(2585.20±161.06)U/L;혈청지방매2 h(446.00±181.54)U/L、4 h(517.40±165.22)U/L、6 h(475.20±170.37)U/L;균수재관주시간연장유명현승고추세(P<0.05),이선조직병리절편정현축점가중적염세포침윤급조직수종(P<0.05)등개변.결론 본모형수술조작간편、우능구교호지모의림상이선결혈재관주적과정,시연구이선결혈재관주손상적교이상동물모형.
Objective To build a convenient model of pancreas ischemia reperfusion injury (IRI)in rats for investigating its negative impact on pancreas. Methods Wistar rats were randomized into 4groups:(1)sham-operated animals with dissociation of the pancreatic tail-segment ( sham, n=20 ); ( 2 )control animals with dissociation of the pancrea-tic tail-segment, 30 seconds of ischemia by bcloking the abdominal aorta and flushing(control, n=20);(3) Group New model animals experienced with dissociation of the pancreatic tail-segment, flushing, 120 mins of ischemia and reperfusion (IR, n=20). The level of serum amylase and lipase, wet to weight ratio of pancreas and pathological changes of pancreas were observed at0, 2, 4, 6 hours after reperfusion. Results The level of serum amylase[2 h (3127.80±150.85) U/L, 4 h (3122.80±131.52) U/L, 6 h (2585.20±161.06) U/L]and lipase[2 h (446.00±181.54) U/L, 4 h (517.40±165.22) U/L, 6 h (475.20 ± 170.37) U/L]in Group New model were increased significantly after perfusion accordingly, The tissue damage of pancreas become more serious after perfusion. Conclusion The model shows a typical IRl on pancreas of rats. It's a ideal and clinically relevant animal model for the study of pancreas IRI after transplantation.