CAJ | 학술논문

目的研究生长激素受体(GHR)在结直肠癌细胞株表面的表达情况并探讨人类重组生长激素(rh-GH)对人类结直肠癌细胞增殖的影响.方法采用流式细胞术及免疫荧光法检测9株人类结直肠癌细胞株表面GHR表达；四甲基偶氮唑蓝比色(MTT)法检测不同浓度rh-GH以及生长激素受体拮抗剂(GHRA)对结直肠癌细胞增殖的影响.结果 GHR在多株结直肠癌细胞株表面存在表达差异,其中GHR在HCT-8人类结直肠癌细胞高表达[(58.23±5.51 )％],LoVo细胞几乎不表达[(0.20±0.04) ％].一定质量浓度的r-hGH( 50、100 ng/ml)对GHR+的HCT-8细胞有促增殖作用(均P＜0.05),但并不呈剂量依赖；应用GHRA封闭GHR后,r-hGH的促增殖作用消失；而rhGH对GHR-的人类结肠癌细胞株LoVo增殖无影响(均P＞0.05).结论外源性生长激素对人类结直肠癌细胞有促增殖作用,rh-GH在结直肠癌患者中的使用需要谨慎.
목적 연구생장격소수체(GHR)재결직장암세포주표면적표체정황병탐토인류중조생장격소(rh-GH)대인류결직장암세포증식적영향.방법 채용류식세포술급면역형광법검측9주인류결직장암세포주표면GHR표체；사갑기우담서람비색(MTT)법검측불동농도rh-GH이급생장격소수체길항제(GHRA)대결직장암세포증식적영향.결과 GHR재다주결직장암세포주표면존재표체차이,기중GHR재HCT-8인류결직장암세포고표체[(58.23±5.51 )％],LoVo세포궤호불표체[(0.20±0.04) ％].일정질량농도적r-hGH( 50、100 ng/ml)대GHR+적HCT-8세포유촉증식작용(균P＜0.05),단병불정제량의뢰；응용GHRA봉폐GHR후,r-hGH적촉증식작용소실；이rhGH대GHR-적인류결장암세포주LoVo증식무영향(균P＞0.05).결론 외원성생장격소대인류결직장암세포유촉증식작용,rh-GH재결직장암환자중적사용수요근신.
Objective To detect the expression of GHR in colorectal cancer cell lines and determine whether recombinant human growth hormone can promote the proliferation of colorectal cancer cells in vitro.Methods GHR distribution was assessed by flow cytometry and immunofluorescence method in 9 colorectal cancer cell lines.The effect of recombinant human growth factor on colorectal cancer cell line proliferation was assessed by MTT method.Results Different GHR expression was determinated in 9 colorectal caner cell lines.GHR was highly expressed in HCT-8 while GHR expression could hardly be detected in LoVo.r-hGH could promote GHR(+) HCT-8 cell proliferation at 50 ng/ml and 100 ng/ml (P ＜0.05).But this effect was not dose dependent.When the neutralizing antibody was used to block GHR activity,this r-hGH dependent proliferation effect was eliminated.r-hGH could not promote GHR (-) LoVo cell proliferation (P ＞0.05).Conclusion The results demonstrates that r-hGH could promote GHR (+) tumor cell proliferation and this effect is mediated by GHR.The use of r-hGH on the colorectal cancer patients should be cautious.