肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2011年
5期
303-306
,共4页
姚苏霞%李丽%杨宣琴%孙瑞芳%王全红%王晓菊
姚囌霞%李麗%楊宣琴%孫瑞芳%王全紅%王曉菊
요소하%리려%양선금%손서방%왕전홍%왕효국
食管肿瘤%受体,表皮生长因子%人类表皮生长因子2%拓扑异构酶Ⅱ
食管腫瘤%受體,錶皮生長因子%人類錶皮生長因子2%拓撲異構酶Ⅱ
식관종류%수체,표피생장인자%인류표피생장인자2%탁복이구매Ⅱ
Esophageal neoplasms%Receptor,epidermal growth factor%Human epidermal growth factor receptor 2%Topoisomerase Ⅱ
目的 探讨表皮生长因子受体(EGFR),人类表皮生长因子2(Her-2),拓扑异构酶Ⅱ(TOPO Ⅱ)在食管上皮癌变过程中的蛋白表达情况与临床病理参数的关系以及三者之间的相互关系.方法 收集107例食管鳞状细胞癌标本,均包括食管正常上皮、上皮内瘤变和鳞状细胞癌.应用组织芯片技术和免疫组织化学EnVision二步法分析EGFR、Her-2、TOPO Ⅱ的蛋白表达.结果 从食管正常鳞状上皮、低级别上皮内瘤变、高级别上皮内瘤变到癌的发展过程中EGFR与TOPO Ⅱ表达率呈现逐步上升的趋势(8.41%、7.94%、27.27%、50.47%;3.74%、4.76%、20.45%、43.93%)(P=0.031).且随着组织学分级的增加二者的表达水平递增(P=0.009),但与性别、年龄、浸润深度、淋巴结转移情况无相关关系.Spearman相关分析显示其二者的表达呈正相关(r1=0.410,P<0.05).Her-2蛋白表达在3组之间差异无统计学意义(P>0.05),与各病理学参数比较差异亦无统计学意义(P>0.05),且与EGFR、TOPO Ⅱ蛋白表达无相关性(r 2=0.112,P>0.05;r3 =0.186,P>0.05).结论 EGFR、TOPOⅡ与食管鳞状细胞癌发生、发展密切相关,尤其在高级别上皮内瘤变阶段其蛋白表达发生质的改变.而Her-2基因在山西地区食管癌发生、发展中作用尚不确定.
目的 探討錶皮生長因子受體(EGFR),人類錶皮生長因子2(Her-2),拓撲異構酶Ⅱ(TOPO Ⅱ)在食管上皮癌變過程中的蛋白錶達情況與臨床病理參數的關繫以及三者之間的相互關繫.方法 收集107例食管鱗狀細胞癌標本,均包括食管正常上皮、上皮內瘤變和鱗狀細胞癌.應用組織芯片技術和免疫組織化學EnVision二步法分析EGFR、Her-2、TOPO Ⅱ的蛋白錶達.結果 從食管正常鱗狀上皮、低級彆上皮內瘤變、高級彆上皮內瘤變到癌的髮展過程中EGFR與TOPO Ⅱ錶達率呈現逐步上升的趨勢(8.41%、7.94%、27.27%、50.47%;3.74%、4.76%、20.45%、43.93%)(P=0.031).且隨著組織學分級的增加二者的錶達水平遞增(P=0.009),但與性彆、年齡、浸潤深度、淋巴結轉移情況無相關關繫.Spearman相關分析顯示其二者的錶達呈正相關(r1=0.410,P<0.05).Her-2蛋白錶達在3組之間差異無統計學意義(P>0.05),與各病理學參數比較差異亦無統計學意義(P>0.05),且與EGFR、TOPO Ⅱ蛋白錶達無相關性(r 2=0.112,P>0.05;r3 =0.186,P>0.05).結論 EGFR、TOPOⅡ與食管鱗狀細胞癌髮生、髮展密切相關,尤其在高級彆上皮內瘤變階段其蛋白錶達髮生質的改變.而Her-2基因在山西地區食管癌髮生、髮展中作用尚不確定.
목적 탐토표피생장인자수체(EGFR),인류표피생장인자2(Her-2),탁복이구매Ⅱ(TOPO Ⅱ)재식관상피암변과정중적단백표체정황여림상병리삼수적관계이급삼자지간적상호관계.방법 수집107례식관린상세포암표본,균포괄식관정상상피、상피내류변화린상세포암.응용조직심편기술화면역조직화학EnVision이보법분석EGFR、Her-2、TOPO Ⅱ적단백표체.결과 종식관정상린상상피、저급별상피내류변、고급별상피내류변도암적발전과정중EGFR여TOPO Ⅱ표체솔정현축보상승적추세(8.41%、7.94%、27.27%、50.47%;3.74%、4.76%、20.45%、43.93%)(P=0.031).차수착조직학분급적증가이자적표체수평체증(P=0.009),단여성별、년령、침윤심도、림파결전이정황무상관관계.Spearman상관분석현시기이자적표체정정상관(r1=0.410,P<0.05).Her-2단백표체재3조지간차이무통계학의의(P>0.05),여각병이학삼수비교차이역무통계학의의(P>0.05),차여EGFR、TOPO Ⅱ단백표체무상관성(r 2=0.112,P>0.05;r3 =0.186,P>0.05).결론 EGFR、TOPOⅡ여식관린상세포암발생、발전밀절상관,우기재고급별상피내류변계단기단백표체발생질적개변.이Her-2기인재산서지구식관암발생、발전중작용상불학정.
Objective To investigate the expression of EGFR, Her-2 and TOPO Ⅱ in esophageal canceration course, analyze the correlation between the expression and clinical pathological parameters and the correlation of the three genes. Methods EGFR, Her-2 and TOPO Ⅱ were detected by Tissue microarray technology and Envision immunohistochemistry method in 107 cases of esophageal carcinoma, including normal esophageal epithelium, esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma. Results The positive expression rates of EGFR and TOPO Ⅱ display an improving trend from normal esophageal epithelium, intraepithelial neoplasia to carcinoma (P =0.031) in the above four groups. The positive expression rates of EGFR were 8.41 %, 7.94 %, 27.27 %, 50.47 %, and TOPO Ⅱ were 3.74 %, 4.76 %, 20.45 %, 43.93 %. Furthermore, the expression showed gradually incresing with histological grades advance (P =0.009). There was no correlation between EGFR or TOPO Ⅱ and gender, age, lymph node or distant metastasis (P >0.05). There was a positive correlation between EGFR and TOPO Ⅱ (r 1=0.410, P <0.05). There were no significance of Her-2 protein expression among the three groups(P >0.05), no correlation was obtained between Her-2 and gender, age, the depth of invasion or lymph node metastasis (P >0.05), no relationship between Her-2 and EGFR or TOPO Ⅱ either. Conclusion EGFR and TOPO Ⅱ are closely related to the occurrence and development of esophageal squamous cell carcinoma, their expressions all make a qualitative change in the esophageal high-grade intraepithelial neoplasia. The role of Her-2 in the development of esophageal squamous cell carcinoma is still not definite in Shanxi province.
