CAJ | 학술논문

以紫外光谱、荧光光谱、粘度法和凝胶电泳方法研究了全反式维甲酸合钇(Ⅲ)配合物与DNA的作用.结果表明,该配合物能在生理条件下比配体和金属离子更有效地切割质粒DNA,体系离子强度和pH值的变化对配合物的切割活性有较大影响,自由基捕捉剂的加入不影响配合物的切割活性.该配合物对DNA的切割可能通过水解机理进行.该配合物可使DNA的粘度增加,使EB-DNA体系的荧光强度和DNA溶液的紫外吸收强度降低.据此推断,该配合物主要以嵌入方式与DNA作用.
이자외광보、형광광보、점도법화응효전영방법연구료전반식유갑산합을(Ⅲ)배합물여DNA적작용.결과표명,해배합물능재생리조건하비배체화금속리자경유효지절할질립DNA,체계리자강도화pH치적변화대배합물적절할활성유교대영향,자유기포착제적가입불영향배합물적절할활성.해배합물대DNA적절할가능통과수해궤리진행.해배합물가사DNA적점도증가,사EB-DNA체계적형광강도화DNA용액적자외흡수강도강저.거차추단,해배합물주요이감입방식여DNA작용.
The interaction of the Y(Ⅲ) complex of all-trans retinoic acid (Y(RA)2·Ac· 8H2O) with DNA has been characterized by UV-absorption and fluorescence spectroscopy, viscosity measurements and agarose gel elecplasmid DNA than that of all-trans retinoic acid and Y3+ at physiological pH and temperature, which may be one that of retinoic acid. It was found that the process of plasmid DNA cleavage was sensitive to ionic strength and pH, however, these radical scavengers almost had no effect on the DNA cleavage reaction. The above results sugreaction. The results of viscosity measurements, UV-absorption and fluorescence characterization on the interac tercalating mode.