CAJ | 학술논문

本研究选用产量性状有显著差异的7个粳稻品种,按照Griffing双列杂交方法Ⅳ配制21个杂交组合,用SSR和SRAP分子标记分析亲本遗传距离及其与粳稻产量性状杂种优势问的关系,并比较分析两种分子标记在估算遗传距离时的差别.结果表明,每对SSR引物产生1～11条多态性带,平均3.8条,而每对SRAP引物组合产生1～15条多态性带,平均5.2条.SRAP引物所扩增的条带数和多态性何点数分别是SSR引物的3.3倍和1.3倍.两种分子标记对遗传相似系数较小的品种进行聚类分析时可获得一致的结果,但对遗传相似系数较大的品种进行聚类分析时所得结果并不一致;粳稻产量性状杂种优势的表现大小因件状和杂交组合不同而异;F_1杂种产量性状的表现与亲本自身的性状特点和互补关系密切,用SSR和SRAP分子标记遗传距离难以预测粳稻杂种后代的产量表现和杂种优势强弱.
본연구선용산량성상유현저차이적7개갱도품충,안조Griffing쌍렬잡교방법Ⅳ배제21개잡교조합,용SSR화SRAP분자표기분석친본유전거리급기여갱도산량성상잡충우세문적관계,병비교분석량충분자표기재고산유전거리시적차별.결과표명,매대SSR인물산생1～11조다태성대,평균3.8조,이매대SRAP인물조합산생1～15조다태성대,평균5.2조.SRAP인물소확증적조대수화다태성하점수분별시SSR인물적3.3배화1.3배.량충분자표기대유전상사계수교소적품충진행취류분석시가획득일치적결과,단대유전상사계수교대적품충진행취류분석시소득결과병불일치;갱도산량성상잡충우세적표현대소인건상화잡교조합불동이이;F_1잡충산량성상적표현여친본자신적성상특점화호보관계밀절,용SSR화SRAP분자표기유전거리난이예측갱도잡충후대적산량표현화잡충우세강약.
In this research, we employed seven cultivars of japonica rice with significant differences in yield traits to be as materials, and made twenty-one hybrid combinations according to Griffing dialed mating system Ⅳ. We analyzed the genetic distances of the test materials based on SSR and SRAP markers and its relationship with heterosis of yield traits, compared and analyzed the difference of genetic distances estimated by using SSR and SRAP markers. The results showed that the number of bands produced per pair of SSR primers ranged from one to eleven, with an average of 3.8 bands per primer, similarly, which of each pair of SRAP primers produced one to fifteen bands,with an average of 5.2. bands per primer. The number of bands and polymorphic locus amplified by using SRAP marker were 3.3 times and 1.3 times more than that of SSR marker, respectively. The cluster analysis of tested lines with low genetic similarity by using SSR and SRAP, which results were coincident, while in the lines with high genetic similarity the situation is quite the reverse. The heterosis of yield traits in japonica rice was up to yield traits and hybrid combination; the yield traits of hybrid F_1 depends on the characteristics and complementary advantages of parents, therefore it is unsuitable to predict the yield and heterosis of hybrid progeny through genetic distance of parents which estimated by using SSR and SRAP markers.