CAJ | 학술논문

目的探讨中国人群高尿酸血症患者TNF-α启动子区-308位点多态性、-238位点基因多态性与HUA及其代谢表型的关联.方法高尿酸血症患者188例,正常人51例,应用聚合酶链反应-限制性片段长度多态性分析方法,检测TNF-a基因启动子区-308G/A单核苷酸多态性位点基因型;尿酸酶法测血尿酸,葡萄糖氧化酶法测快速血糖.比色法测总胆同醇和甘油三酯,高密度脂蛋白胆固醇由选择性抑制法测定.上述化验均在血样提取后3h内完成.-20℃保存血样以便测定胰岛素浓度,免疫放射测定法测得胰岛素.随机选择高尿酸血症患者35例,正常人39例,同样方法检测TNF-α基因启动子区-238G/A单核苷酸多态性位点基因型及各生化指标.结果对两位点单核苷酸多态性分析发现,高尿酸血症组-308位点AA+GA分布频率(23.94%)明显高于对照组(11.76%)(P=0.0001);-238位点的GG和GA基因型分布频率在两组之间无明显差异(P=0.08).同时,-308位点的GA+AA基因型组和GG型组相比,其中腰臀比、收缩期和舒张期血压、尿酸、甘油三酯差别均有统计学意义(P=0.05～0.01);-238位点的两组基因型问各检测指标差别均无意义.结论本研究结果显示HUA个体中TNF-α启动子区-308A携带者的基因型与高尿酸血症及其代谢表型有关.
목적 탐토중국인군고뇨산혈증환자TNF-α계동자구-308위점다태성、-238위점기인다태성여HUA급기대사표형적관련.방법 고뇨산혈증환자188례,정상인51례,응용취합매련반응-한제성편단장도다태성분석방법,검측TNF-a기인계동자구-308G/A단핵감산다태성위점기인형;뇨산매법측혈뇨산,포도당양화매법측쾌속혈당.비색법측총담동순화감유삼지,고밀도지단백담고순유선택성억제법측정.상술화험균재혈양제취후3h내완성.-20℃보존혈양이편측정이도소농도,면역방사측정법측득이도소.수궤선택고뇨산혈증환자35례,정상인39례,동양방법검측TNF-α기인계동자구-238G/A단핵감산다태성위점기인형급각생화지표.결과 대량위점단핵감산다태성분석발현,고뇨산혈증조-308위점AA+GA분포빈솔(23.94%)명현고우대조조(11.76%)(P=0.0001);-238위점적GG화GA기인형분포빈솔재량조지간무명현차이(P=0.08).동시,-308위점적GA+AA기인형조화GG형조상비,기중요둔비、수축기화서장기혈압、뇨산、감유삼지차별균유통계학의의(P=0.05～0.01);-238위점적량조기인형문각검측지표차별균무의의.결론 본연구결과현시HUA개체중TNF-α계동자구-308A휴대자적기인형여고뇨산혈증급기대사표형유관.
Objective To investigate the correlation between the-308 site,-238 site gene polymorphism of TNF-αa promoter region in hypemricemh and its metabolic phenotype.Methods HUA patients 188,normal people(NHU)51,the-308G/A site single nu-cleofide polymorphism of TNF-a gene promoter region was decided by Polymerase chain reaction-restriction fragment length polymor-phism(PCR-RFLP)method.The level of serum uric acid is detected by uricase.blood glucose by glucose oxidase.Selective inhibition assay for total cholesterol,triglyceride and high-density lipoprotein cholesterol.All these tests were complted within three hours afterextraction.Keep blood samples at-20℃ to detect the concentration of insulin using immunoradiometrie assay.HUA patients 35.NHU 39,the -238G/A site single nucleofide polymorphism of TNF-a gene promoter region was decided by the same method.Results By smdying two single nucleotide polymorphism,the GA+AA frequency of-308 site(23.94%)in HUA was significantly higher than NHU(11.76%)(P=0.0001);the genotype frequencies of GA and GG in-238 site had no significant difference between the two groups(P=0.08).Meanwhile,comparing GA+AA genotype and CG group of-308 site,waist-hip ratio,systolic and diastolic blood pressure,uric acid,triglycerides differences were statistically significant(P=0.05-0.01);the difference in-238 site between the two genotypes waft no significance.Conclusion The results of this study show-308A carriers of TNF-α promoter region in HUA individual is correlated with hyperuficemia and its metabolic phenotype.