CAJ | 학술논문

采用改良的CTAB法提取陆地棉总RNA,运用RT-PCR技术首次克隆了陆地棉类烯醇酶基因的CDS序列,该基因已经在GenBank上登录,登录号为 EU169604,其开放阅读框为1338 bp,编码445个氨基酸残基.对其序列和进化分析表明,陆地棉烯醇酶基因与其它植物中的烯醇酶基因有较高的相似性,该基因在进化过程中是相当保守的.半定量RT-PCR表达分析结果显示该基因在陆地棉的叶、根、茎中均有表达,但在根中的表达量高于叶和茎.
채용개량적CTAB법제취륙지면총RNA,운용RT-PCR기술수차극륭료륙지면류희순매기인적CDS서렬,해기인이경재GenBank상등록,등록호위 EU169604,기개방열독광위1338 bp,편마445개안기산잔기.대기서렬화진화분석표명,륙지면희순매기인여기타식물중적희순매기인유교고적상사성,해기인재진화과정중시상당보수적.반정량RT-PCR표체분석결과현시해기인재륙지면적협、근、경중균유표체,단재근중적표체량고우협화경.
The total RNA of Gossypium hirsutum was extracted by modified CTAB method and the open reading frame of enolase gene from Gossypium hirsutum was cloned by RT-PCR,named GhEno , which was 1338 bp long and encoded 445 amino acids.The GhEno gene was registered in GenBank with the access number of EU169604.Sequence analysis alignment indicated that GhEno gene had high homology with those of other plants.The phylogenetic tree analysis showed that the GhEno was clustered in the same group with Hevea brasiliensis and Ricinus communis .Semi-quantity RT-PCR analysis indicated that expression of GhEno was detected in roots, stems and leaves,however it was stronger in roots than in leaves and stems.