CAJ | 학술논문

将大孔树脂HP-2MGL和交联葡聚糖凝胶SephadexLH-20用于葡萄籽原花青素的纯化.HP-2MGL可除去蛋白、多糖、高聚物等杂质,获得原花青素含量为96.5%的GSPP3.SephadexLH.20能够进一步纯化GSPP3,获得低聚原花青素GSPP3-SP1和GSPP3-SP2.经HPLC柱分离,GSPP3-SP1有4个峰,GSPP3-SP2有7个峰.GSPP3-SP1和GSPP3-SP2质谱分析发现,含有大量m/z287、m/z289、m/z290、m/z408、m/z426、m/z577、m/z578、m/z580等分子离子及碎片质谱信息,证明是由儿荣素和表儿茶素构成的原花青素二聚体的同分异构体.激光光散射与GPC联用测定原花青素粗提物GSP的重均相对分子质量为4.15×10~4,多分散系数为1.65;GSPP3的重均相对分子质量为4.26×10~3,平均聚合度为13,多分散系数为1.56,相对分子质量分布曲线呈对称分布,以高聚合度原花青素为主;葡萄籽原花青素纯化分级级分GSPP3-SP的重均相对分子质量为1.89×10~3,甲均聚合度为6,多分散系数为1.45,以低聚合度原花青素为主.
장대공수지HP-2MGL화교련포취당응효SephadexLH-20용우포도자원화청소적순화.HP-2MGL가제거단백、다당、고취물등잡질,획득원화청소함량위96.5%적GSPP3.SephadexLH.20능구진일보순화GSPP3,획득저취원화청소GSPP3-SP1화GSPP3-SP2.경HPLC주분리,GSPP3-SP1유4개봉,GSPP3-SP2유7개봉.GSPP3-SP1화GSPP3-SP2질보분석발현,함유대량m/z287、m/z289、m/z290、m/z408、m/z426、m/z577、m/z578、m/z580등분자리자급쇄편질보신식,증명시유인영소화표인다소구성적원화청소이취체적동분이구체.격광광산사여GPC련용측정원화청소조제물GSP적중균상대분자질량위4.15×10~4,다분산계수위1.65;GSPP3적중균상대분자질량위4.26×10~3,평균취합도위13,다분산계수위1.56,상대분자질량분포곡선정대칭분포,이고취합도원화청소위주;포도자원화청소순화분급급분GSPP3-SP적중균상대분자질량위1.89×10~3,갑균취합도위6,다분산계수위1.45,이저취합도원화청소위주.
Macroporous resin HP-2MGL and SephadexLH-20 Gel was used to purify the proanthocyanidin from grape seed.Fraction GSPP3HP-2MGL was obtained by separation of crude grape seed extract through HP-2MGL and its proanthocyanidin content reached 96.5%. The fraction was further purified with SephadexLH-20 and two proanthocyanidin dipolymers GSPP3-SP1 and GSPP3-SP2 were resulted. HPLC analysis showed 4 peaks from GSPP3-SP1 and 7 peaks from GSPP3-SP2 were recognized. Mass spectrums revealed the abundance of the fragment ions like m/z 287, m/z 289, m/z 290, m/z 408, m/z 426,m/z 577, m/z 578 and m/z 580, suggesting this proanthocyanidin was a catechuic acid epigallocatechin-epicatechin dimer. The Mw of GSP was determined to be 4.15 × 10~4 by laser light scattering combined with GPC and the coefficient of polydispersity was 1.65. The Mw of GSPP3 was 4.26 ×10~3 with the average degree of polymerization of 13 and the coefficient of polydispersity of 1.56. The relative molecular weight curve showed a symmetrical distribution and this proanthocyanidin was highly polymerized.GSPP3-SP was found to lowly polymerized proanthocyanidin with the Mw of 1.89×10~3, the average degree of polymerization of 6 and the coefficient of polydispersity of 1.45.