CAJ | 학술논문

建立了固相萃取/高效液相色谱法测定肝癌细胞中多西紫杉醇浓度的方法.细胞样品经三氯乙酸沉淀蛋白,Bond-elut C_(18)固相萃取柱提取,采用Agilent TC-C_(18)(5 μm,4.6 mm×150 mm)色谱柱分析,以乙腈-0.02 mol/L醋酸铵缓冲液(体积比50 : 50,醋酸调至pH 5.0)为流动相,流速为1 mL/min,检测波长为230 nm,进样体积为20 μL.多西紫杉醇的质量浓度在0.05 ～2.25 mg/L范围内线性关系良好,相关系数r=0.999 3,检出限为0.03 mg/L,提取回收率为93% ～95%,其日内、日间精密度(n=5)不大于6.9%,且稳定性良好.方法简便、快速、灵敏度高、干扰小,可用于生物样品中低浓度多西紫杉醇的测定.
건립료고상췌취/고효액상색보법측정간암세포중다서자삼순농도적방법.세포양품경삼록을산침정단백,Bond-elut C_(18)고상췌취주제취,채용Agilent TC-C_(18)(5 μm,4.6 mm×150 mm)색보주분석,이을정-0.02 mol/L작산안완충액(체적비50 : 50,작산조지pH 5.0)위류동상,류속위1 mL/min,검측파장위230 nm,진양체적위20 μL.다서자삼순적질량농도재0.05 ～2.25 mg/L범위내선성관계량호,상관계수r=0.999 3,검출한위0.03 mg/L,제취회수솔위93% ～95%,기일내、일간정밀도(n=5)불대우6.9%,차은정성량호.방법간편、쾌속、령민도고、간우소,가용우생물양품중저농도다서자삼순적측정.
To establish a high performance liquid chromatographic(HPLC) method for determination of docetaxel in hepatocellular cancer cells.Docetaxel was extracted from hepatocellular cancer cells by a Bond-elut C_(18) solid phase extraction(SPE)column,and separated on a Agilent TC-C_(18)(5 μm,4.6 mm×150 mm) column by using mobile phase consisted of ammonium acetate buffer(0.02 mol/L,pH 5.0)- acetonitrile(50 : 50,by volume) with a flow rate of 1 mL/min at detection wavelength of 230 nm.The result indicated that the calibration of docetaxel was linear in the range of 0.05-2.25 mg/L with correlation coefficient of 0.999 3.The detection limit for docetaxel was 0.03 mg/L.The extraction recoveries of docetaxel form forin cells sample ranged from 93% to 95%.The within-day RSDs(n=5) and the between-day RSDs(n=5) were both not more than 6.9%.The method was convenient,accurate and sensitive,and could be applied in the determination of low level of docetaxel in biological samples.