CAJ | 학술논문

对不同荔枝核样品采用香草醛-高氯酸比色法测定荔枝核总皂甙含量,用硝酸铝-亚硝酸钠比色法测定总黄酮含量,用蒽酮-硫酸法测定多糖成分的含量;结果表明:不同荔枝核样品中总皂甙含量在1.30%～1.40%之间,总黄酮含量在5.78%～6.80%之间;多糖2.85%～3134%之间.以芦丁为对照,从对不同体系产生的超氧阴离子自由基、羟自由基和DPPH自由基的清除能力及脂质过氧化的抑制活性等方面研究荔枝核提取物的抗氧化活性.结果显示:荔枝核提取物质最浓度为0.8mg/ml时,对超氧阴离子自由基的清除率达92.96%,超过同质量浓度的芦丁;质量浓度为0.5mg/ml时,对羟自由基的清除率达85.56%,略低于相应对照.荔枝核提取物对脂质过氧化的抑制率随浓度的增加而增大,质量浓度为0.5mg/ml时,抑制率达52.56%,稍低于相应对照.妃子笑荔枝核提取物质量浓度为1mg/ml时,其清除DPPH自由基的能力超过同质量浓度的芦丁.
대불동려지핵양품채용향초철-고록산비색법측정려지핵총조대함량,용초산려-아초산납비색법측정총황동함량,용은동-류산법측정다당성분적함량;결과표명:불동려지핵양품중총조대함량재1.30%～1.40%지간,총황동함량재5.78%～6.80%지간;다당2.85%～3134%지간.이호정위대조,종대불동체계산생적초양음리자자유기、간자유기화DPPH자유기적청제능력급지질과양화적억제활성등방면연구려지핵제취물적항양화활성.결과현시:려지핵제취물질최농도위0.8mg/ml시,대초양음리자자유기적청제솔체92.96%,초과동질량농도적호정;질량농도위0.5mg/ml시,대간자유기적청제솔체85.56%,략저우상응대조.려지핵제취물대지질과양화적억제솔수농도적증가이증대,질량농도위0.5mg/ml시,억제솔체52.56%,초저우상응대조.비자소려지핵제취물질량농도위1mg/ml시,기청제DPPH자유기적능력초과동질량농도적호정.
Total saponins, flavonoids and polysaccharides in litchi seed extract (LSE) of different cultivars were determined using vanillin-perchloric acid method, aluminum nitrate-sodium nitrite method and anthrone-sulfuric acid method, respectively, with their contents varying 1.30% - 1.40%, 5.78% - 6.80%, 2.85% - 3.34%, respectively. Superoxide anion radical, hydroxyl radical and DPPH free radical scavenging abilities of LSE were evaluated and compared with that of rutin. Result show that superoxide anion scavenging rate was up to 92.96% (LSE concentraction 0.8mg/ml), higher than that of rutin of the same concentration, and hydroxyl radical scavenging rate was 85.56% (LSE concentraction 0.5mg/ml), and DPPH free radical scavenging rate was 52.56% (LSE concentration 0.5mg/ml). LSE inhibited lipid peroxidation with the inhibition rate up to 52.56% (LSE concentration 0.5mg/ml).