CAJ | 학술논문

采用自行研制的非接触电导检测器,结合毛细管电泳技术,以10mmol/L的2-N-吗啡啉乙磺酸(MES)/L-组氨酸(L-His)为缓冲溶液,系统研究了缓冲溶液浓度、pH值、样品浓度对电渗流峰的变化规律.结果表明:随着样品KC1溶液的浓度的减小,电渗流峰出现由正峰逐渐变为负峰的现象,并结合非接触电导检测原理、L-His的特性和质子平衡方程进行了的解释.
채용자행연제적비접촉전도검측기,결합모세관전영기술,이10mmol/L적2-N-마배람을광산(MES)/L-조안산(L-His)위완충용액,계통연구료완충용액농도、pH치、양품농도대전삼류봉적변화규률.결과표명:수착양품KC1용액적농도적감소,전삼류봉출현유정봉축점변위부봉적현상,병결합비접촉전도검측원리、L-His적특성화질자평형방정진행료적해석.
The influence of sample concentration and buffer solution on electroosmotic flow (EOF) was studied by capilly electrophoresis with contactless conductivity detection, in which 2-(N-Morpholino) ethanesulfonic acid L-histidine was used as the separation buffer solution. The EOF peak transfered from positive to negative with the decrease of the concentration of KCl solution. This phenomenon could be explained with the principle of contactless conductivity detection and proton ionization equilibria of L-His.