中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2012年
7期
864-868
,共5页
脱细胞基质%脱细胞巩膜%眼部修复
脫細胞基質%脫細胞鞏膜%眼部脩複
탈세포기질%탈세포공막%안부수복
Acellular tissue matrix%Acellular sclera%Eyelid reconstruction
目的 将交联型异体脱细胞巩膜植入眼睑,研究其组织相容性及抗原性变化.方法 将20只新西兰大白兔随机平均分为两组,去除睑板后,分别植入兔交联型脱细胞巩膜和异体巩膜,于植入后1、4、8、12周观察全身及局部光镜和免疫学变化.结果 脱细胞巩膜和异体巩膜植入眼睑后第1周、4周、8周,两组间外周血IgG、IgA、IgM、C3、C4和CD4+T细胞、CD8+T细胞定量均无明显差异.术后1周、4周、8周、12周,植入局部的光镜显示:脱细胞巩膜组的炎症反应和淋巴细胞浸润要小于异体巩膜组,8周后植入的异体巩膜组织中央部发生少量坏死溶解现象,术后12周两组成纤维细胞及血管完全长入植片.术后1、4、8周,局部CD4+T及CD8+T细胞计数显示脱细胞巩膜组明显少于异体巩膜组.结论 兔脱细胞巩膜植入眼睑后引起的免疫反应较同种异体巩膜小.
目的 將交聯型異體脫細胞鞏膜植入眼瞼,研究其組織相容性及抗原性變化.方法 將20隻新西蘭大白兔隨機平均分為兩組,去除瞼闆後,分彆植入兔交聯型脫細胞鞏膜和異體鞏膜,于植入後1、4、8、12週觀察全身及跼部光鏡和免疫學變化.結果 脫細胞鞏膜和異體鞏膜植入眼瞼後第1週、4週、8週,兩組間外週血IgG、IgA、IgM、C3、C4和CD4+T細胞、CD8+T細胞定量均無明顯差異.術後1週、4週、8週、12週,植入跼部的光鏡顯示:脫細胞鞏膜組的炎癥反應和淋巴細胞浸潤要小于異體鞏膜組,8週後植入的異體鞏膜組織中央部髮生少量壞死溶解現象,術後12週兩組成纖維細胞及血管完全長入植片.術後1、4、8週,跼部CD4+T及CD8+T細胞計數顯示脫細胞鞏膜組明顯少于異體鞏膜組.結論 兔脫細胞鞏膜植入眼瞼後引起的免疫反應較同種異體鞏膜小.
목적 장교련형이체탈세포공막식입안검,연구기조직상용성급항원성변화.방법 장20지신서란대백토수궤평균분위량조,거제검판후,분별식입토교련형탈세포공막화이체공막,우식입후1、4、8、12주관찰전신급국부광경화면역학변화.결과 탈세포공막화이체공막식입안검후제1주、4주、8주,량조간외주혈IgG、IgA、IgM、C3、C4화CD4+T세포、CD8+T세포정량균무명현차이.술후1주、4주、8주、12주,식입국부적광경현시:탈세포공막조적염증반응화림파세포침윤요소우이체공막조,8주후식입적이체공막조직중앙부발생소량배사용해현상,술후12주량조성섬유세포급혈관완전장입식편.술후1、4、8주,국부CD4+T급CD8+T세포계수현시탈세포공막조명현소우이체공막조.결론 토탈세포공막식입안검후인기적면역반응교동충이체공막소.
Objective To find a method of acellular sclera preparation,investigate the histocompatibility of acellular sclera implanted in rabbit eyelid and antigenic changes between sclera and acellular sclera. Methods Acellular sclera was prepared with 2％ Triton X-100,DNase and RNase.Twenty New-Zealand white rabbits (20 right eyes) were divided into two groups randomly.Sclera and the acellular sclera were implanted the big tarsus with 3 mm X 10 mm in upper eyelid in two groups.The appearances,immunologic changes and inflammatory reaction of eyelids were observed with quantifying the immunoglobulins and complements,flow cytometry,HE stain and immunofluorescence assay in 1,4,8,and 12 weeks postoperation. Results Acelullar sclera could be successful prepared with 2％ Triton X-100.There were no statistical differences between two groups in IgG,IgA,IgM,C3,C4,CD4+T cells and CD8+T cells of blood (P ＞0.05).In the 1st week,4th week and 8th week,the average numbers of CD4+T cells and CD8+T cells in eyelid of sclera group were 25±7 and 11±4,83±27 and 28±9,69±24 and 22±7,while those of acelllar sclera group were 13±5 and 7±4,26±11 and 13±21,7±13 and 7±3 respectively.The inflammatory cells and lymphocytes in sclera were much more than those in acellular sclera. Conclusions Acellular sclera is easy to be prepared and can be used to repair the eyelid.
