中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2012年
7期
732-735
,共4页
李明学%王权%王宾%严东旺%唐华美%彭志海%于振海
李明學%王權%王賓%嚴東旺%唐華美%彭誌海%于振海
리명학%왕권%왕빈%엄동왕%당화미%팽지해%우진해
胃肿瘤%胃肠富集Krüppel样因子4%预后
胃腫瘤%胃腸富集Krüppel樣因子4%預後
위종류%위장부집Krüppel양인자4%예후
Stomach neoplasms%Krüppel-like factor 4%Prognosis
目的 探讨KLF4在胃癌组织中的表达及其与胃癌患者预后的关系.方法 收集上海交通大学附属第一人民医院2004-2009年胃癌根治性手术标本264例,应用实时定量PCR和免疫组织化学染色方法检测KLF4在胃癌及癌旁组织中的表达.结果 KLF4蛋白在胃癌和癌旁组织中的阳性表达率分别为43.2％(114/264)和79.2％(209/264),差异有统计学意义(P＜0.01).KLF4表达与浸润深度(P＜0.05)、淋巴结转移(P＜0.01)、远处转移(P＜0.05)及临床分期(P＜0.05)有关.KLF4阳性与阴性表达患者5年总体生存率分别为65.2％和41.2％,5年无瘤生存率分别为50.4％和36.2％,差异均有统计学意义(P＜0.05).KLF4表达是胃癌患者总体生存(HR=2.89,95％CI:1.18～9.23)和无瘤生存(HR=2.14,95％CI:1.03～4.37)的独立预后因素.结论 KLF4在胃癌组织中的表达显著下调;KLF4有可能成为预测胃癌患者预后的生物学标志物.
目的 探討KLF4在胃癌組織中的錶達及其與胃癌患者預後的關繫.方法 收集上海交通大學附屬第一人民醫院2004-2009年胃癌根治性手術標本264例,應用實時定量PCR和免疫組織化學染色方法檢測KLF4在胃癌及癌徬組織中的錶達.結果 KLF4蛋白在胃癌和癌徬組織中的暘性錶達率分彆為43.2％(114/264)和79.2％(209/264),差異有統計學意義(P＜0.01).KLF4錶達與浸潤深度(P＜0.05)、淋巴結轉移(P＜0.01)、遠處轉移(P＜0.05)及臨床分期(P＜0.05)有關.KLF4暘性與陰性錶達患者5年總體生存率分彆為65.2％和41.2％,5年無瘤生存率分彆為50.4％和36.2％,差異均有統計學意義(P＜0.05).KLF4錶達是胃癌患者總體生存(HR=2.89,95％CI:1.18～9.23)和無瘤生存(HR=2.14,95％CI:1.03～4.37)的獨立預後因素.結論 KLF4在胃癌組織中的錶達顯著下調;KLF4有可能成為預測胃癌患者預後的生物學標誌物.
목적 탐토KLF4재위암조직중적표체급기여위암환자예후적관계.방법 수집상해교통대학부속제일인민의원2004-2009년위암근치성수술표본264례,응용실시정량PCR화면역조직화학염색방법검측KLF4재위암급암방조직중적표체.결과 KLF4단백재위암화암방조직중적양성표체솔분별위43.2％(114/264)화79.2％(209/264),차이유통계학의의(P＜0.01).KLF4표체여침윤심도(P＜0.05)、림파결전이(P＜0.01)、원처전이(P＜0.05)급림상분기(P＜0.05)유관.KLF4양성여음성표체환자5년총체생존솔분별위65.2％화41.2％,5년무류생존솔분별위50.4％화36.2％,차이균유통계학의의(P＜0.05).KLF4표체시위암환자총체생존(HR=2.89,95％CI:1.18～9.23)화무류생존(HR=2.14,95％CI:1.03～4.37)적독립예후인소.결론 KLF4재위암조직중적표체현저하조;KLF4유가능성위예측위암환자예후적생물학표지물.
Objective To explore the expression of gut-enriched Krüippel-like factor 4 (KLF4)in gastric cancer,and its association with prognosis.Methods Surgical specimens were collected from 264 patients undergoing radical surgery between 2004 and 2009 in the Affiliated Qianfoshan Hospital,Shandong University.KLF4 mRNA level of specimens was detected by real-time PCR.KLF4 protein expression was measured by immunohistochemistry on tissue microarray,which contained primary gastric cancer,corresponding para-cancerous tissue,and paired lymph node metastases.Results Real-time PCR revealed that mRNA level of KLF4 was down-regulated in gastric cancer compared with paired normal gastric mucosa.Immunohistochemistry on tissue microarray showed gastric cancer tissues had significantly lower KLF4 levels compared with paired normal gastric tissues. By univariate and multivariate analysis,KLF4 was a significant predictor of survival and recurrence.Conclusion KLF4 expression is significantly down-regulated in gastric cancer,and is an independent predictor of survival and recurrence.
