CAJ | 학술논문

目的探讨江苏汉族人群蛋白激酶cη(PRKCH)基因1425G/A单核苷酸多态与脑梗死的关系.方法采用病例-对照的方法,选取255例脑梗死患者和225例其他疾病患者或健康体检者为对照,应用PCR扩增产物直接测序法筛查PRKCH基因1425G/A单核苷酸多态,对相关资料编码后输入SPSS 13.0统计学软件进行数据分析.结果在脑梗死组中GA+AA基因型频率(56.86%)和A等位基因频率(36.27%)明显高于对照组(44.44%和24.67%;χ2=7.377,P=0.007;χ2=15.104,P<0.01);对脑梗死组的不同亚型进一步分析发现,腔隙性脑梗死亚组该位点基凶型(63.09%)和等位摹因频率(40.27%)分布与对照组(44.44%和24.67%)相比,差异有统计学意义(χ2=11.744,P=0.01;χ2=20.445,P<0.01).Logistic回归分析显示,高血压、糖尿病、高血脂和A等位基因是江苏汉族人群腔隙性脑梗死的独立危险因素.结论 PRKCH基因1425G/A多态可能与脑梗死发生相关,尤其是与腔隙性脑梗死的发生相关.
목적 탐토강소한족인군단백격매cη(PRKCH)기인1425G/A단핵감산다태여뇌경사적관계.방법 채용병례-대조적방법,선취255례뇌경사환자화225례기타질병환자혹건강체검자위대조,응용PCR확증산물직접측서법사사PRKCH기인1425G/A단핵감산다태,대상관자료편마후수입SPSS 13.0통계학연건진행수거분석.결과 재뇌경사조중GA+AA기인형빈솔(56.86%)화A등위기인빈솔(36.27%)명현고우대조조(44.44%화24.67%;χ2=7.377,P=0.007;χ2=15.104,P<0.01);대뇌경사조적불동아형진일보분석발현,강극성뇌경사아조해위점기흉형(63.09%)화등위모인빈솔(40.27%)분포여대조조(44.44%화24.67%)상비,차이유통계학의의(χ2=11.744,P=0.01;χ2=20.445,P<0.01).Logistic회귀분석현시,고혈압、당뇨병、고혈지화A등위기인시강소한족인군강극성뇌경사적독립위험인소.결론 PRKCH기인1425G/A다태가능여뇌경사발생상관,우기시여강극성뇌경사적발생상관.
Objective To explore the association between 1425G/A single nucleotide polymorphism(SNP)of protein kinase Cη gene(PRKCH)and cerebral infarction in Jiangsu Han population.Methods 255 patients with cerebral infarction and 225 controls were recruited in our case-control study.The 1425G/A in PRKCH gene was detected by direct sequencing of PCR products.Data were coded and entered in SPSS Windows(version 13.0).Results The frequencies of the GA+AA genotypes(56.86%)and A allele (36.27%)in cerebral infarct group were significantly higher than those in control group(44.44%and24.67%.χ2=7.377,P=0.007 and χ2=15.104,P<0.01).Further analysis indicated that the genotypes(63.09%)and alleles(40.27%)frequencies were statistically different between lacunar infarction subtype and controls(44.44%and 24.67%;χ2=11.744,P=0.Ol and χ2=20.445,P<0.01).Logistic regression analysis revealed that hypertension,diabetes mellitus.hyperlipidemia and the A allele of 1425G/A polymorphism were independent risky factors for lacunar infarction.Conclusions The SNP 1425G/A in PRKCH is closely associated with cerebral infarction.particularly with lacunar infarction.