CAJ | 학술논문

目的观察贲门癌手术患者机体免疫状态的变化.方法采用流式细胞术检测97例贲门癌患者T淋巴细胞亚群(Th1、Th2、CD3+、CD4+、CD8+细胞),采用双抗体夹心酶联免疫吸附试验( ELISA)检测血清中白细胞介素(IL)-2、干扰素(IFN)-γ、IL-4、IL-10、可溶性白细胞介素-2受体R(sIL-2R)和肿瘤坏死因子(TNF)-β水平.结果贲门癌患者术前外周血Th1/Th2细胞比值(0.43±0.13)较正常对照组(0.58±0.12)降低(P＜0.05),其Th1细胞表达的IL-2、IFN-γ等细胞因子水平低于正常对照组；Th2细胞表达的IL-4、IL-10细胞因子水平高于正常对照组(P＜0.05).T细胞介导的细胞免疫及血清sIL-2R和TNF-β水平与肿瘤TNM分期相关,其特征为:CD3+、CD4+细胞减少(P＜0.05),而CD8+细胞增加(P＜ 0.01)；CD4 +/CD8+细胞比值下降(P＜0.01),血清sIL-2R和TNF-β水平升高(p＜0.05) 贲门癌根治术后1个月CD3+、CD4+细胞回升,CD8+细胞回降,同时血清sIL-2R和TNF-β水平降低(P＜0.01).结论贲门癌患者外周血Th1/Th2细胞比值降低,细胞因子表达失衡,患者存在免疫功能抑制.
목적 관찰분문암수술환자궤체면역상태적변화.방법 채용류식세포술검측97례분문암환자T림파세포아군(Th1、Th2、CD3+、CD4+、CD8+세포),채용쌍항체협심매련면역흡부시험( ELISA)검측혈청중백세포개소(IL)-2、간우소(IFN)-γ、IL-4、IL-10、가용성백세포개소-2수체R(sIL-2R)화종류배사인자(TNF)-β수평.결과 분문암환자술전외주혈Th1/Th2세포비치(0.43±0.13)교정상대조조(0.58±0.12)강저(P＜0.05),기Th1세포표체적IL-2、IFN-γ등세포인자수평저우정상대조조；Th2세포표체적IL-4、IL-10세포인자수평고우정상대조조(P＜0.05).T세포개도적세포면역급혈청sIL-2R화TNF-β수평여종류TNM분기상관,기특정위:CD3+、CD4+세포감소(P＜0.05),이CD8+세포증가(P＜ 0.01)；CD4 +/CD8+세포비치하강(P＜0.01),혈청sIL-2R화TNF-β수평승고(p＜0.05) 분문암근치술후1개월CD3+、CD4+세포회승,CD8+세포회강,동시혈청sIL-2R화TNF-β수평강저(P＜0.01).결론 분문암환자외주혈Th1/Th2세포비치강저,세포인자표체실형,환자존재면역공능억제.
Objective To observing the immune status changing in cardia carcinoma post-operation patients.Methods Flow cytometry was used to detect T lymphocyte subsets (Th1,Th2,CD3 +,CD4 +,CD8 + ) in 97 cardia carcinoma patients,Enzyme linked immunosorbent assay (ELISA) assay was used to analyze the serum status of interleukin (IL)-2,interferon (IFN)-γ,IL-4,IL-10,soluble interleukin 2 receptor (sIL-2R) and tumor necrosis factor (TNF)-β.Results Peripheral blood Th1/Th2 ratio was significantly lower in pre-operation patients (0.43 ±0.13) than normal controls(0.58 ±0.12) ( P ＜0.05 ).cytokines status as IL-2 and IFN-γ expressed by Th1 was significantly lower than control group,while IL-4 and IL-10 expressed by Th2 was significantly higher (P ＜ 0.05).T cell mediated cell immunity has changed and disordered.The degree has related to TNM stage,The characteristics were:CD3 + and CD4 + were decreased and CD8 + increased( P ＜ 0.05 ) ; the Th1/Th2 ratio was significantly decreased ( P ＜ 0.05 ),sIL-2R and TNF-β were increased ( P ＜ 0.05 ).1 month post-operation,CD3 +,CD4 + were increased and CD8 + decreased,sIL-2R and TNF-β deaeased ( P ＜ 0.01 ).Conclusion Th1/Th2 cell ratio decreased in cardia carcinoma patients,cytokine expression imbalance in patients with suppressed immune function.