中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
10期
1523-1525
,共3页
精索静脉曲张%生精上皮%凋亡%HIF-α%bax
精索靜脈麯張%生精上皮%凋亡%HIF-α%bax
정색정맥곡장%생정상피%조망%HIF-α%bax
Varicocele%Seminiferous epithelium%Apoptosis%HIF-1α%bax
目的 观察高压氧(HBO)对精索静脉曲张(VC)SD大鼠生精细胞凋亡的影响,探讨精索静脉曲张导致男性不育的机制.方法 50只青春期雄性SD大鼠,随机抽出10只作为假手术对照组(A).A组只游离左肾静脉不予结扎,其余40只部分结扎左肾静脉建立VC模型,8周后,将建立了VC模型的40只随机分为VC模型组(B)和HBO干预的VC模型组(C),C组用HBO对VC模型进行干预.实验结束后各组大鼠切取双侧睾丸,采用原位末端标记法(TUNEL)检测大鼠睾丸生精细胞凋亡,免疫组织化学检测低氧诱导因子(HIF)-α、bcl-2及bax的基因表达.结果 B组较A组生精细胞凋亡数明显增多,HIF-α、bax表达增强(P＜0.01),bcl-2表达减弱(P＜0.01),bcl-2/bax比值降低;C组大鼠较B组生精细胞凋亡数减少(P＜0.01),HIF-α表达减弱(P＜0.01)、bcl-2/bax比值上升,而与A组比较差异无统计学意义.结论 VC大鼠生精细胞凋亡率增加,HIF-α表达增强、bcl-2和bax基因表达失调为其凋亡机制之一;高压氧可能通过HIF-α、bcl-2和bax基因调控途径调节生精细胞凋亡,从而改善VC所致的生精功能障碍.
目的 觀察高壓氧(HBO)對精索靜脈麯張(VC)SD大鼠生精細胞凋亡的影響,探討精索靜脈麯張導緻男性不育的機製.方法 50隻青春期雄性SD大鼠,隨機抽齣10隻作為假手術對照組(A).A組隻遊離左腎靜脈不予結扎,其餘40隻部分結扎左腎靜脈建立VC模型,8週後,將建立瞭VC模型的40隻隨機分為VC模型組(B)和HBO榦預的VC模型組(C),C組用HBO對VC模型進行榦預.實驗結束後各組大鼠切取雙側睪汍,採用原位末耑標記法(TUNEL)檢測大鼠睪汍生精細胞凋亡,免疫組織化學檢測低氧誘導因子(HIF)-α、bcl-2及bax的基因錶達.結果 B組較A組生精細胞凋亡數明顯增多,HIF-α、bax錶達增彊(P＜0.01),bcl-2錶達減弱(P＜0.01),bcl-2/bax比值降低;C組大鼠較B組生精細胞凋亡數減少(P＜0.01),HIF-α錶達減弱(P＜0.01)、bcl-2/bax比值上升,而與A組比較差異無統計學意義.結論 VC大鼠生精細胞凋亡率增加,HIF-α錶達增彊、bcl-2和bax基因錶達失調為其凋亡機製之一;高壓氧可能通過HIF-α、bcl-2和bax基因調控途徑調節生精細胞凋亡,從而改善VC所緻的生精功能障礙.
목적 관찰고압양(HBO)대정색정맥곡장(VC)SD대서생정세포조망적영향,탐토정색정맥곡장도치남성불육적궤제.방법 50지청춘기웅성SD대서,수궤추출10지작위가수술대조조(A).A조지유리좌신정맥불여결찰,기여40지부분결찰좌신정맥건립VC모형,8주후,장건립료VC모형적40지수궤분위VC모형조(B)화HBO간예적VC모형조(C),C조용HBO대VC모형진행간예.실험결속후각조대서절취쌍측고환,채용원위말단표기법(TUNEL)검측대서고환생정세포조망,면역조직화학검측저양유도인자(HIF)-α、bcl-2급bax적기인표체.결과 B조교A조생정세포조망수명현증다,HIF-α、bax표체증강(P＜0.01),bcl-2표체감약(P＜0.01),bcl-2/bax비치강저;C조대서교B조생정세포조망수감소(P＜0.01),HIF-α표체감약(P＜0.01)、bcl-2/bax비치상승,이여A조비교차이무통계학의의.결론 VC대서생정세포조망솔증가,HIF-α표체증강、bcl-2화bax기인표체실조위기조망궤제지일;고압양가능통과HIF-α、bcl-2화bax기인조공도경조절생정세포조망,종이개선VC소치적생정공능장애.
Objective To study the effects of hyperbaric oxygenation (HBO) on experimental varicocele on the apoptosis of spermatogenic cell and the expression of hypoxia-inducible factor-1 α( HIF-1α),bax,bcl-2 in adolescent rats. Methods Experimental varicocele models were created by partial ligation of left renal vein in adolescent Sprague-Dawley (SD) male rats. A total of 50 rats were randomly divided into 3 groups, namely sham operation group ( A, n = 10 ) , varicocele group ( B, n = 20 ), and HBO administered after being induced varicocele model ( C, n = 20 ). All the animals were killed 8w after operation. The left testis was collected and the expression of HIF-1α, bcl-2 and bax was detected by immunohistochemical method. The apoptosis of spermatogenic cells was detected by in situ terminal deoxylnucleotidyl transferase mediated-dUTP nick end labeling (TUNEL) technique. Results The expression of HIF-1α and bax in left testis of varicocele group was significantly higher than that of A and C group ( P ＜ 0. 01 ) and positive correlation was found between them ( r = 0. 6751, P ＜ 0. 05 ). The expression of bcl-2 in left testis of varicocele group was significantly lower than that of A and C group ( P ＜ 0.05 ). The incidence of apoptosis was decreased significantly in C group than that in B group (P ＜ 0. 01 ), and the apoptosis number of spermatogenic cells of bilateral testes was decreased. Conclusion HBO can change the expression of HIF-1α, bcl-2and apoptosis associated gene bax in germ cells of experimental varicocele adolescent rats. The apoptosis of spermatogenic cells of bilateral testes caused by left varicocele may be one of the mechanisms of male sterility caused by varicocele.
