CAJ | 학술논문

目的探讨超短波对鼠断尾再植后血管危象的影响及作用机制. 方法制备断尾再植模型:除尾侧静脉、尾骨保留,断离尾,吻合尾动脉.80只3月龄健康SD大鼠随机分4组:空白组(0组)、模型组(1组)、对照组(2组)和超短波(USW)组(3组).3组又分高剂量组(3A组)、低剂量组(3B组).0组结扎尾动脉,其余组均吻合.1组正常管理；2组术后5d天每天1次予罂粟碱液；3组术后5d每日1次予局部吻合口处USW治疗20 min:3A组USW剂量为3档,50mA,3B组剂量为2档,28 mA.观察鼠尾成活情况10 d.分组前测鼠尾吻合口近、远侧皮温差1次,每日测1次.分组前和术后第8h,测血浆NO. 结果鼠尾成活率:断尾组0％,断尾再植组44％,P=0.002;断尾再植的各组(1、2、3A、3B组)间成活率比较:3B组＜2组＜1组＜3A组,P=0.022；3B组与1组比较,P=0.031；2组与1组比较,P=0.221.皮温差变化量术后较术前比较:术后第1天:3A组＜1组,P=0.028;术后第6天:3A组＜3B组＜1组＜2组,P=0.024;术后第7天:3B组＜1组＜3A组＜2组,P=0.001.NO术后较术前比较:3B组＞3A组＞2组＞1组＞0组,P=0.000. 结论实验大鼠断尾再植模型可行;USW治疗能提高鼠断尾再植存活率,在术后第1、6、7天,能减小皮温差,提高术后第8hNO浓度,防治血管危象.
목적 탐토초단파대서단미재식후혈관위상적영향급작용궤제. 방법 제비단미재식모형:제미측정맥、미골보류,단리미,문합미동맥.80지3월령건강SD대서수궤분4조:공백조(0조)、모형조(1조)、대조조(2조)화초단파(USW)조(3조).3조우분고제량조(3A조)、저제량조(3B조).0조결찰미동맥,기여조균문합.1조정상관리；2조술후5d천매천1차여앵속감액；3조술후5d매일1차여국부문합구처USW치료20 min:3A조USW제량위3당,50mA,3B조제량위2당,28 mA.관찰서미성활정황10 d.분조전측서미문합구근、원측피온차1차,매일측1차.분조전화술후제8h,측혈장NO. 결과 서미성활솔:단미조0％,단미재식조44％,P=0.002;단미재식적각조(1、2、3A、3B조)간성활솔비교:3B조＜2조＜1조＜3A조,P=0.022；3B조여1조비교,P=0.031；2조여1조비교,P=0.221.피온차변화량술후교술전비교:술후제1천:3A조＜1조,P=0.028;술후제6천:3A조＜3B조＜1조＜2조,P=0.024;술후제7천:3B조＜1조＜3A조＜2조,P=0.001.NO술후교술전비교:3B조＞3A조＞2조＞1조＞0조,P=0.000. 결론 실험대서단미재식모형가행;USW치료능제고서단미재식존활솔,재술후제1、6、7천,능감소피온차,제고술후제8hNO농도,방치혈관위상.
Objective To research the effect mechanism of ultrashortwave in the correlation of ultrashortwave and the tail replantation, provide the experiment basis of clinical practice of prevention and cure for the vascular crisis after micromodule anastomosis. Methods Eighty Sprague-Dawley(SD) rats of clean grade were 3-month-old,female,and were divided into four groups:control group (group 0),model group (group 1 ),contrast group (group 2),ultrashortwave (USW) group (group 3).The preparation of tail replantation model was cut off soft tissue except for caudal veins on both sides of the tail. The coccyx was not broken away from tail.At last,the audal artery under abdominal main centre ditch was anastomosed.In experiment process, the USW group was divided into high dosage group (group 3A) and low dosage group (group 3B). The caudal arterys were ligated and not anastomosed in the group 0. Caudal arterys in other groups were anastomosed.Rats in the group 0 and group 1 received no treatment,normal management after the operation. Rats in the group 2 were given abdominal cavity injection of papaverin liquid immediately,then once a day to 5 days after the tail replantation.Rats in the group 3 were immediately given USW therapy of twenty minutes on the anastomosis section,and then once a day for 5 days after the tail replantation.The USW dosage of group 3A was 3th grade and 50mA. The USW dosage of group 3B was 2th grade and 28mA.The survival rate of the rat tails was observed after the tail replantation for 10 day.Before being grouped,it was measured that the tail skin temperature diference between near and far side of anastomosis section.After the tail relpantation, the temperature diference was inspected daily for 10 postoperative days hence. Before rats were grouped and the eighth hour after the tail replantation, rats were collected blood plasma specimens and measured contents of nitric oxide with destination colorimetric mathods of nitric oxide. Results Carrying out comparison of survival rate of every group,the output weve:between tail cutting off group (group 0,0) and tail replantation group (group 1,2,3,43.94％) to compare P ＞ 0.05; between each group of the tail replantation groups (1,2,3A,3B group) to compare P＞ 0.05,group 3B ＜ 2 ＜ 1 ＜ 3A; between group 3B and group 1 to compare P ＞ 0.05; between group 2 and group 1 to compare P ＞ 0.05. Each group were compared with the change daily between postoperative and preceding operative the skin temperature diference,single-factor analysis of variance (One-Way ANOVA) analysis:Postoperative 1 day,group 3A ＜ 1,P ＜ 0.05.Postoperative 6 day:3A ＜ 3B ＜ 1 ＜ 2,P ＞ 0.05.Postoperative 7 day:group 3B ＜ 1 ＜ 3A ＜ 2,P ＜ 0.05.Each group were compared with the change of the content of nitric oxide between postoperative 8 hour and preceding operative,with rank-sum test:group 3B ＞ 3A ＞ 2 ＞ 1 ＞ 0,H =33.760,P ＜ 0.05,shows statistically significant.Conclusions USW therapy,especially USW low-dose therapy,can reduce vascular crisis and improve the survival rate of replanted rat tails,after the postoperative 1,6,7 days,reduce skin temperature,improve blood supply,improve nitric oxide at postoperative eighth hour,prevent vascular crisis.Rat tail replantation model in this experiment is feasible.