CAJ | 학술논문

研究了N源对产朊假丝酵母细胞生长和谷胱甘肽(GSH)合成的影响.在此基础上,分别以(NH_4)_2SO_4和尿素作为单一N源,摇瓶条件下研究了不同C、N比对GSH发酵的影响.结果发现尿素有利于细胞生长,而(NH_4)_2SO_4更有利于GSH的合成,并且酵母细胞在利用这2种N源合成GSH时,各自具有最佳的C、N比((NH_4)_2SO_4为8.3 mol/mol,尿素为5.6 mol/mol).最佳C、N比下的GSH分批发酵结果显示,尿素是更合适的N源,最终细胞干质量和GSH产量可以分别达到16.48 g/L和246.4 mg/L.最后分别采用发酵动力学模型和代谢网络分析对该结果产生的原因进行了定量解释.
연구료N원대산원가사효모세포생장화곡광감태(GSH)합성적영향.재차기출상,분별이(NH_4)_2SO_4화뇨소작위단일N원,요병조건하연구료불동C、N비대GSH발효적영향.결과발현뇨소유리우세포생장,이(NH_4)_2SO_4경유리우GSH적합성,병차효모세포재이용저2충N원합성GSH시,각자구유최가적C、N비((NH_4)_2SO_4위8.3 mol/mol,뇨소위5.6 mol/mol).최가C、N비하적GSH분비발효결과현시,뇨소시경합괄적N원,최종세포간질량화GSH산량가이분별체도16.48 g/L화246.4 mg/L.최후분별채용발효동역학모형화대사망락분석대해결과산생적원인진행료정량해석.
The effect of nitrogen sources on cell growth and GSH production by Candida utilis in shaking flasks was studied. (NH_4)_2SO_4 and urea were individually chosen as the single nitrogen source at varied C to N ratio. Urea was beneficial to cell growth while (NH_4)_2SO_4 was favorable to GSH production. The optimal C/N molar ratio were 8.3 and 5.6 for (NH_4)_2SO_4 and urea. Batch fermentation of GSH using the single nitrogen source was conducted under the optimal C/N ratios. The maximum dry cell weight and GSH concentration of 16.48 g/L and 246.4 mg/L were achieved at the optimized conditions. The parameters in kinetics, metabolite flux and metabolic network were also discussed.