CAJ | 학술논문

目的通过对人脑胶质瘤U87细胞中CD133+和CD133-细胞进行配对研究,寻找差异性蛋白.方法通过磁珠选择分离U87中CD133+和CD133-细胞,利用双向凝胶电泳(2D - PAGE)检测上述细胞总体蛋白,分析差异性蛋白点.通过质谱( MALDI -TOF - MS)对蛋白点进行鉴定,建立两种细胞差异性蛋白文库.结果 CD133+和CD133-细胞配对比较,蛋白质组学检测结果中,差异有统计学意义(P＜0.05)的2D - PAGE蛋白点共有73个,＞1.5倍差异蛋白点有46个,＞2倍差异蛋白点有27个,＞3倍差异蛋白点有8个.在CD133+ U87细胞中,有10个蛋白表达增多(上调),63个蛋白表达下降(下调).共得到44张高质量质谱肽质量指纹图谱(PMF),鉴定了35种蛋白质.结论 CD133+和CD133-细胞是同一细胞株中两种不同的细胞,蛋白方面存在的差异,将成为进一步研究它们相互关系的重要靶点.
목적 통과대인뇌효질류U87세포중CD133+화CD133-세포진행배대연구,심조차이성단백.방법 통과자주선택분리U87중CD133+화CD133-세포,이용쌍향응효전영(2D - PAGE)검측상술세포총체단백,분석차이성단백점.통과질보( MALDI -TOF - MS)대단백점진행감정,건립량충세포차이성단백문고.결과 CD133+화CD133-세포배대비교,단백질조학검측결과중,차이유통계학의의(P＜0.05)적2D - PAGE단백점공유73개,＞1.5배차이단백점유46개,＞2배차이단백점유27개,＞3배차이단백점유8개.재CD133+ U87세포중,유10개단백표체증다(상조),63개단백표체하강(하조).공득도44장고질량질보태질량지문도보(PMF),감정료35충단백질.결론 CD133+화CD133-세포시동일세포주중량충불동적세포,단백방면존재적차이,장성위진일보연구타문상호관계적중요파점.
Objective To analyze differences between CD133+ and CD133- glioma U87 cells by methods of proteomics.Methods 2D - PAGE was performed to separate the proteins of the CD133 + and CD133 - U87 cells,which were isolated by magnetic cell sorting.The differential protein spots were analyzed by software analysis,subject to in- gel digestion,and identified by matrix assistant laser desorption time -of - flight mass spectrometry ( MALDI - TOF - MS) and MALDI - TOF/TOF - MS.Results There were 73 differentially expressed protein spots observed in comparing between CD133 + and CD133- U87 cells by 2D -PAGE ( P ＜ 0.05 ).Spots with ＞ 1.5 - fold expression changes were 46,and ＞ 2 - fold were 27,and ＞ 3 -fold were 8.Ten spots were increased,while 63 spots were decreased in CD133 + U87 cells.Forty -four high -quality peptide mass fingerprinting and 35 proteins were obtained by MS. Conclusions There are significant differences between CD133+ and CD133- U87 cells.Differentially proteins have been obtained by proteomics analysis,which are important targets to the study of brain cancer stem cells (BTSCs).