CAJ | 학술논문

目的莱姆病螺旋体的基因型和临床表现、疫苗菌株和抗原菌株的选择存在密切的关系，所以对中国菌株进行分子流行病学研究，可为莱姆病的防治提供科学依据。方法 5S～23S rRNA基因间隔区RFLP分析和16+23S rRNA基因RFLP分析。结果中国菌株至少被分为3个基因型(B.burgdorferi sensu stricto、B.garinii和B.afzelii)，B.garinii和B.afzelii占优势，B.burgdorferi sensu stricto少见。少数菌株用上述方法尚不能明确其分类地位，需进一步研究，中国很可能存在世界上独特的新基因型。结论中国菌株的基因型明显不同于北美菌株，而同欧洲菌株比较接近。5S～23S rRNA基因间隔区RFLP分析方法简便、快速、准确，是理想的基因型分类方法，可作为国内菌株基因型鉴定的常规方法。
목적래모병라선체적기인형화림상표현、역묘균주화항원균주적선택존재밀절적관계，소이대중국균주진행분자류행병학연구，가위래모병적방치제공과학의거。방법 5S～23S rRNA기인간격구RFLP분석화16+23S rRNA기인RFLP분석。결과중국균주지소피분위3개기인형(B.burgdorferi sensu stricto、B.garinii화B.afzelii)，B.garinii화B.afzelii점우세，B.burgdorferi sensu stricto소견。소수균주용상술방법상불능명학기분류지위，수진일보연구，중국흔가능존재세계상독특적신기인형。결론중국균주적기인형명현불동우북미균주，이동구주균주비교접근。5S～23S rRNA기인간격구RFLP분석방법간편、쾌속、준학，시이상적기인형분류방법，가작위국내균주기인형감정적상규방법。
Objective　There is a close relationship between genospecies and clinical manifestation, the choice of strain for vaccine and serodiagnosis antigen. So molecular epidemiology of Borrelia burgdorferi sensu lato in China is important for the prevention of Lyme disease. Methods　Rrf-rrl intergenic spacer RFLP analysis and 16+23S rRNA gene RFLP analysis. Results　There were at least three genospecies in China. The most of them belongs to B. garinii and B. afzelii. A few isolates showed unique RFLP pattern. Their taxonomic positions remain unclear. There are probably new genospecies in China. Conclusions　Chinese strains are quite different from that of Northern America and similar to that in Europe. The method of rrf-rrl intergenic spacer RFLP analysis is simple and accurate, so it could be used as routine genotyping method in China.