CAJ | 학술논문

目的　建立一种检测血清汉滩病毒抗体IgM的方法　。方法　将汉滩病毒抗原点于硝基纤维膜上，应用φ 15nm的羊抗人IgM金标抗体与血清标本中的汉滩病毒IgM结合，再加银加强试剂，阳性者为棕黑色。结果　整个试验过程需要10 min，用该法与ELISA法对比检测临床标本，结果　有良好的一致性，总符合率为98％。结论　银强化滴金免疫法操作简单，试剂稳定、安全、敏感、快速，适于基层。
목적　건립일충검측혈청한탄병독항체IgM적방법　。방법　장한탄병독항원점우초기섬유막상，응용φ 15nm적양항인IgM금표항체여혈청표본중적한탄병독IgM결합，재가은가강시제，양성자위종흑색。결과　 정개시험과정수요10 min，용해법여ELISA법대비검측림상표본，결과　유량호적일치성，총부합솔위98％。결론　 은강화적금면역법조작간단，시제은정、안전、민감、쾌속，괄우기층。
Objective To establish a more rapid and simple method for detecting anti - Hantavirus IgM in pa-tient's serum. Methods A silver enhanced dot irvunogold filtration assay(SEDIGFA)was developed by coating thenucleocapsid protein of Hantavirus on nitrocellulose (NC) membrance,adding colloidal gold labeled sheep anti - humanIgM antibody and the silver enhancer. The whole procedure can be finished Within 10 minutes. Results The coinci-dence rate of the resuhs of serum sarnples simultaneously operated with ELISA and SEDEIGFA was 98% for anti - Han-tavirus IgM Conchusions SEDIGFA is more simple and rapid for application to small medical laboratories. It can beapplied for early diagnosis of hemorrhagic fever with renal syndrome(HFRS).