中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2009年
7期
431-434
,共4页
裴豪%陆忠华%钱金娟%杨小娟%蒋祥虎%曹立森
裴豪%陸忠華%錢金娟%楊小娟%蔣祥虎%曹立森
배호%륙충화%전금연%양소연%장상호%조립삼
肝炎,乙型,慢性%CD4阳性T淋巴细胞%T淋巴细胞,调节性%T淋巴细胞,细胞毒性%肝
肝炎,乙型,慢性%CD4暘性T淋巴細胞%T淋巴細胞,調節性%T淋巴細胞,細胞毒性%肝
간염,을형,만성%CD4양성T림파세포%T림파세포,조절성%T림파세포,세포독성%간
Hepatitis B,chronic%CD4-positive T-lymphocytes%T-lymphocytes,regulatory%T-lymphocytes,cytotoxic%Liver
目的 研究CD4+CD25+调节性T细胞和HBV特异性CTL在慢性乙型肝炎患者外周血和肝组织中的表达和临床意义.方法 流式细胞分析技术和流式细胞术细胞因子测定法(CFC)检测157例HBV感染者(包括急性乙型肝炎20例、慢性乙型肝炎115例、乙型肝炎肝硬化22例)和20例健康对照组外周血和部分肝组织中CD4+CD25+调节性T细胞和HBV特异性CTL的表达.组间分析采用t检验.结果急性乙型肝炎,慢性乙型肝炎轻、中、重度患者外周血中CD4+CD25+调节性T细胞分别为(2.87±0.94)%、(3.53±1.56)%、(4.59±2.98)%和(3.65±1.73)%,明显高于对照组的(2.36±0.60)%(t值分别为2.04、5.97、3.30和3.17,P<0.01);慢性乙型肝炎轻、中、重度和乙型肝炎肝硬化患者外周血HBV特异性CTL为(0.189土0.152)%、(0.103±0.110)%、(0.118±0.120)%和(0.098±0.101)%,明显低于急性乙型肝炎患者的(0.815±0.360)%(t值分别为10.09、11.87、9.17和8.96,P<0.01).肝组织中CD4+CD25+调节性T细胞和HBV特异性CTL的表达高于外周血.结论 CD4+CD25+调节性T细胞可能通过抑制CD8+T淋巴细胞在机体抗病毒过程中发挥重要作用.
目的 研究CD4+CD25+調節性T細胞和HBV特異性CTL在慢性乙型肝炎患者外週血和肝組織中的錶達和臨床意義.方法 流式細胞分析技術和流式細胞術細胞因子測定法(CFC)檢測157例HBV感染者(包括急性乙型肝炎20例、慢性乙型肝炎115例、乙型肝炎肝硬化22例)和20例健康對照組外週血和部分肝組織中CD4+CD25+調節性T細胞和HBV特異性CTL的錶達.組間分析採用t檢驗.結果急性乙型肝炎,慢性乙型肝炎輕、中、重度患者外週血中CD4+CD25+調節性T細胞分彆為(2.87±0.94)%、(3.53±1.56)%、(4.59±2.98)%和(3.65±1.73)%,明顯高于對照組的(2.36±0.60)%(t值分彆為2.04、5.97、3.30和3.17,P<0.01);慢性乙型肝炎輕、中、重度和乙型肝炎肝硬化患者外週血HBV特異性CTL為(0.189土0.152)%、(0.103±0.110)%、(0.118±0.120)%和(0.098±0.101)%,明顯低于急性乙型肝炎患者的(0.815±0.360)%(t值分彆為10.09、11.87、9.17和8.96,P<0.01).肝組織中CD4+CD25+調節性T細胞和HBV特異性CTL的錶達高于外週血.結論 CD4+CD25+調節性T細胞可能通過抑製CD8+T淋巴細胞在機體抗病毒過程中髮揮重要作用.
목적 연구CD4+CD25+조절성T세포화HBV특이성CTL재만성을형간염환자외주혈화간조직중적표체화림상의의.방법 류식세포분석기술화류식세포술세포인자측정법(CFC)검측157례HBV감염자(포괄급성을형간염20례、만성을형간염115례、을형간염간경화22례)화20례건강대조조외주혈화부분간조직중CD4+CD25+조절성T세포화HBV특이성CTL적표체.조간분석채용t검험.결과급성을형간염,만성을형간염경、중、중도환자외주혈중CD4+CD25+조절성T세포분별위(2.87±0.94)%、(3.53±1.56)%、(4.59±2.98)%화(3.65±1.73)%,명현고우대조조적(2.36±0.60)%(t치분별위2.04、5.97、3.30화3.17,P<0.01);만성을형간염경、중、중도화을형간염간경화환자외주혈HBV특이성CTL위(0.189토0.152)%、(0.103±0.110)%、(0.118±0.120)%화(0.098±0.101)%,명현저우급성을형간염환자적(0.815±0.360)%(t치분별위10.09、11.87、9.17화8.96,P<0.01).간조직중CD4+CD25+조절성T세포화HBV특이성CTL적표체고우외주혈.결론 CD4+CD25+조절성T세포가능통과억제CD8+T림파세포재궤체항병독과정중발휘중요작용.
Objective To study the presence of CD4+CD25+regulatory T cells and hepatitis B virus(HBV)specific cytotoxie T lymphocyte(CTL)in peripheral blood and liver tissues of patients with chronic hepititis B(CHB)and its clincial significance.Methods One hundred and fifty-seven HBV-infected patients,including 20 cases of acute hepatitis B,115 cases of chronic hepatitis B,and 22cases of HBV-related liver cirrhosis,and 20 healthy controls were enrolled in this study.Peripheral blood was collected and liver tissues were obtained from some of the enrolled subjects.The CD4+CD25+regulatory T cells and HBV specific CTL were analyzed using flow cytometry and cytokine flow cytometry(CFC).The comparison between groups was done by t test.Results The percentages of CD4+CD25+ regulatory T cells in the peripheral blood of patients with acute hepatitis B and CHB of mild,moderate and severe degree were(2.87±0.94)%,(3.53±1.56)%,(4.59±2.98)%and(3.65±1.73)%,respectively,which were higher than that of controls(2.36±0.60)%(t=2.04,5.97,3.30 and 3.17,respectively,P<0.01).The percentages of HBV specific CTL in the peripheral blood of patients with mild,moderate and severe degree of CHB and HBV-related liver cirrhosis were (0.189±0.152)%,(0.103±0.110)%,(0.118±0.120)%and(0.098±0.101)%,respectively,which were significantly lower than that of acute hepatitis patients [(0.815±0.360)%](t=10.09,11.87,9.17 and 8.96,respectively,P<0.01).CD4+CD25+ regulatory T cells and HBV specific CTL in liver tissues were both higher than those in the peripheral blood.Conclusion CD4+CD25+regulatory T cells may play an important role in anti-HBV immune response through inhibiting CD8+T eell function.
