CAJ | 학술논문

目的了解浙江省狂犬病疫区野生宿主动物种类及感染状况.方法采集浙江省丽水市和杭州市淳安县猫、鼬獾、黑线姬鼠、跳麂、野猪脑组织标本共160份,分别取大脑、中脑、小脑和海马回部位组织,用直接免疫荧光试验(DFA)检测狂犬病病毒特异性抗原和RT-PCR方法检测狂犬病病毒特异性核酸,确定阳性标本.结果脑组织抗原印片经特异性抗狂犬病病毒核蛋白(NV)单克隆抗体免疫荧光染色后在荧光显微镜下可见大量的苹果绿荧光颗粒;狂犬病病毒NP特异性目的基因Nested-PCR得到与预期结果大小一致的扩增产物,两种方法同时检出狂犬病病毒阳性的野生宿主动物脑组织标本5份,其中鼬獾脑组织阳性标本4份,阳性率为8.33%(4/48);黑线姬鼠脑组织阳性标本1份,阳性率为1.75%(1/57);猫、跳麂、野猪脑组织未检出阳性标本.结论在浙江省生物多样性的山区检测出狂犬病病毒感染阳性的野生动物鼬獾和野鼠,从病原学角度说明鼬獾和野鼠可能是人间狂犬病的传染来源和狂犬病病毒的宿主动物.
목적 료해절강성광견병역구야생숙주동물충류급감염상황.방법 채집절강성려수시화항주시순안현묘、유환、흑선희서、도궤、야저뇌조직표본공160빈,분별취대뇌、중뇌、소뇌화해마회부위조직,용직접면역형광시험(DFA)검측광견병병독특이성항원화RT-PCR방법검측광견병병독특이성핵산,학정양성표본.결과 뇌조직항원인편경특이성항광견병병독핵단백(NV)단극륭항체면역형광염색후재형광현미경하가견대량적평과록형광과립;광견병병독NP특이성목적기인Nested-PCR득도여예기결과대소일치적확증산물,량충방법 동시검출광견병병독양성적야생숙주동물뇌조직표본5빈,기중유환뇌조직양성표본4빈,양성솔위8.33%(4/48);흑선희서뇌조직양성표본1빈,양성솔위1.75%(1/57);묘、도궤、야저뇌조직미검출양성표본.결론 재절강성생물다양성적산구검측출광견병병독감염양성적야생동물유환화야서,종병원학각도설명유환화야서가능시인간광견병적전염래원화광견병병독적숙주동물.
Objective To understand the prevalence of rabies among wild animals and the animal species in rabies epidemic areas of Zhejiang province. Methods One hundred and sixty samples were collected from the brain tissues of cats, stoats, Apodemus agrarius, Moschus chinensis, and Sus scrofa in Lishui and Chunan cities of Zhejiang province. Each sample was divided into four parts: cerebrum,mesencephal, cerebellum and cyrus hippocampi which were used to determine the positive samples by detection of rabies virus specific antigens and nucleotides, using DFA and RT-PCR methods. Results Positive slides in the tests contained a glaring, apple green brilliance fluorescence using rabies virus specific monoclonal antibody against nucleoprotein. Using Nested-PCR method targeted at part of N gene,five positive samples were identified which consisting of four positive samples from stoats with positive ratio as 8.33% (4/48) and one positive sample from Apodemns agrarius with positive ratio as 1.75% (1/57). However, no positive result was found from cats, Moschus chinensis, and Sus scrofa samples. Conclusion Rabies virus positive samples were identified from stoats and Apodemus agrarins in the mountain areas with biological diversity in Lishui and Chunan cities of Zhejiang province,indicating that stoats and Apodemus agrarius might have played a role in human rabies and acted as host of rabies virus. In order to effectively prevent and control rabies virus under these complicated geographical and ecological environment, we must understand and evaluate the infection situation among animals in these regions.