中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2008年
5期
499-501,513
,共4页
孙玉兰%胡义德%钱海洪%杨永峰
孫玉蘭%鬍義德%錢海洪%楊永峰
손옥란%호의덕%전해홍%양영봉
辐射%线粒体DNA%凋亡%细胞周期阻滞%基因表达
輻射%線粒體DNA%凋亡%細胞週期阻滯%基因錶達
복사%선립체DNA%조망%세포주기조체%기인표체
Irradiation%Mitochondrial DNA%Apoptosis%Cell cycle arrest%Gent expression
目的 研究X射线辐射对人肺腺癌A549细胞增殖及线粒体DNA(mtDNA)基因表达的影响.方法 8 MV X射线以4 Gy的吸收剂量照射A549细胞,照射后不同时间检测细胞周期分布和凋亡率,电镜下观察细胞超微结构,人mtDNA基因表达谱芯片检测靶基因的表达变化.结果 照射后12 h即出现G2/M期的阻滞,凋亡率在照射后48 h达到峰值,透射电镜下可见凋亡的形态改变.X射线照射后mtDNA编码基因表达呈普遍下调,包括12 h组的1种tRNA和4种mRNA基因、24 h组的2种tRNA和4种mRNA基因、48 h组的13种mRNA和2种rRNA及4种tRNA基因、72 h组的11种mRNA和2种rRNA基因.结论 X射线辐射可导致A549细胞G2/M期阻滞,mtDNA编码基因的普遍低表达可能和辐射诱导凋亡相关.
目的 研究X射線輻射對人肺腺癌A549細胞增殖及線粒體DNA(mtDNA)基因錶達的影響.方法 8 MV X射線以4 Gy的吸收劑量照射A549細胞,照射後不同時間檢測細胞週期分佈和凋亡率,電鏡下觀察細胞超微結構,人mtDNA基因錶達譜芯片檢測靶基因的錶達變化.結果 照射後12 h即齣現G2/M期的阻滯,凋亡率在照射後48 h達到峰值,透射電鏡下可見凋亡的形態改變.X射線照射後mtDNA編碼基因錶達呈普遍下調,包括12 h組的1種tRNA和4種mRNA基因、24 h組的2種tRNA和4種mRNA基因、48 h組的13種mRNA和2種rRNA及4種tRNA基因、72 h組的11種mRNA和2種rRNA基因.結論 X射線輻射可導緻A549細胞G2/M期阻滯,mtDNA編碼基因的普遍低錶達可能和輻射誘導凋亡相關.
목적 연구X사선복사대인폐선암A549세포증식급선립체DNA(mtDNA)기인표체적영향.방법 8 MV X사선이4 Gy적흡수제량조사A549세포,조사후불동시간검측세포주기분포화조망솔,전경하관찰세포초미결구,인mtDNA기인표체보심편검측파기인적표체변화.결과 조사후12 h즉출현G2/M기적조체,조망솔재조사후48 h체도봉치,투사전경하가견조망적형태개변.X사선조사후mtDNA편마기인표체정보편하조,포괄12 h조적1충tRNA화4충mRNA기인、24 h조적2충tRNA화4충mRNA기인、48 h조적13충mRNA화2충rRNA급4충tRNA기인、72 h조적11충mRNA화2충rRNA기인.결론 X사선복사가도치A549세포G2/M기조체,mtDNA편마기인적보편저표체가능화복사유도조망상관.
Objective To investigate the proliferation and expression of mitochondrial DNA(mtDNA) genes in A549 human lung adenocarcinoma induced by X-ray irradiation. Methods A549 cells were irradiated with 8 MV X-ray at the absorbed dose of 4 Gy, followed by incubating the cells for different time. Cell cycle distribution and apoptotic rates were determined by flow cytometry (FCM). The apoptotic morphology was observed by transmission electron microscope (TEM), and the differential expression of mtDNA genes were detected with mtDNA oligonucleotide microarray. Results G2/M phase arrest was manifested 12 h after exposure and the apoptotic rates reached the climax at 48 h incubation. The morphological characters of apoptosis were observed. 1 tRNA genes and 4 mRNA genes, 2 tRNA genes and 4 mRNA genes, 13 mRNA genes and 2 rRNA genes and 4 tRNA genes, 11 mRNA genes and 2 rRNA genes, which were all encoded by mtDNA, were identified down-regulated at 12,24,48 and 72 h, respectively. Conclusions X-ray irradiation can cause cell cycle arrest at the G2/M phase and the down-regulated expression of mitochondrial DNA genes may play a role in radiation-induced apoptosis.
