中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2009年
5期
325-328
,共4页
杨士勇%韩天权%时开网%蔡劬%姜志宏%蒋兆彦%蔡杏兴%张圣道
楊士勇%韓天權%時開網%蔡劬%薑誌宏%蔣兆彥%蔡杏興%張聖道
양사용%한천권%시개망%채구%강지굉%장조언%채행흥%장골도
胆结石%肝脏受体类似物1%基因表达%三磷酸腺苷结合盒(ABC)转运体家族成员abcg5/8
膽結石%肝髒受體類似物1%基因錶達%三燐痠腺苷結閤盒(ABC)轉運體傢族成員abcg5/8
담결석%간장수체유사물1%기인표체%삼린산선감결합합(ABC)전운체가족성원abcg5/8
Cholelithasis%Liver receptor homolog 1%Gene expression%abcg5/8
目的 研究胆囊胆固醇结石病人肝脏的肝脏受体类似物1(Liver receptor homolog 1,LRH-1)及其调控的三磷酸腺苷结合盒(ABC)转运体家族成员abcg5/8的表达,探讨胆固醇结石病发病的机制.方法 27例胆囊胆固醇结石病人,男6例,女21例;年龄平均52岁.10例无胆石症的胆囊息肉病人为对照,男6例,女4例;平均年龄48岁.测定胆汁脂类成分和计算胆汁胆固醇饱和指数.实时定量PCR法测定肝脏LRH-1及abcg5/8 mRNA的表达量.结果 胆石组LRH-1表达高于对照组(14.18±1.80vs7.22±2.22,P<0.05),胆石组肝脏abcg基因mRNA表达量均较对照组增高(abcg5:49.34±3.68 vs 33.48±2.77,P<0.05)abcg8:38.93±5.70 vs 18.70±3.42,P<0.05).胆石组胆汁呈胆固醇过饱和(1.17±0.02).结论 该研究结果提示人类肝脏LRH-1及其调控的abcg5/8的表达增高与胆囊胆固醇结石形成有关.
目的 研究膽囊膽固醇結石病人肝髒的肝髒受體類似物1(Liver receptor homolog 1,LRH-1)及其調控的三燐痠腺苷結閤盒(ABC)轉運體傢族成員abcg5/8的錶達,探討膽固醇結石病髮病的機製.方法 27例膽囊膽固醇結石病人,男6例,女21例;年齡平均52歲.10例無膽石癥的膽囊息肉病人為對照,男6例,女4例;平均年齡48歲.測定膽汁脂類成分和計算膽汁膽固醇飽和指數.實時定量PCR法測定肝髒LRH-1及abcg5/8 mRNA的錶達量.結果 膽石組LRH-1錶達高于對照組(14.18±1.80vs7.22±2.22,P<0.05),膽石組肝髒abcg基因mRNA錶達量均較對照組增高(abcg5:49.34±3.68 vs 33.48±2.77,P<0.05)abcg8:38.93±5.70 vs 18.70±3.42,P<0.05).膽石組膽汁呈膽固醇過飽和(1.17±0.02).結論 該研究結果提示人類肝髒LRH-1及其調控的abcg5/8的錶達增高與膽囊膽固醇結石形成有關.
목적 연구담낭담고순결석병인간장적간장수체유사물1(Liver receptor homolog 1,LRH-1)급기조공적삼린산선감결합합(ABC)전운체가족성원abcg5/8적표체,탐토담고순결석병발병적궤제.방법 27례담낭담고순결석병인,남6례,녀21례;년령평균52세.10례무담석증적담낭식육병인위대조,남6례,녀4례;평균년령48세.측정담즙지류성분화계산담즙담고순포화지수.실시정량PCR법측정간장LRH-1급abcg5/8 mRNA적표체량.결과 담석조LRH-1표체고우대조조(14.18±1.80vs7.22±2.22,P<0.05),담석조간장abcg기인mRNA표체량균교대조조증고(abcg5:49.34±3.68 vs 33.48±2.77,P<0.05)abcg8:38.93±5.70 vs 18.70±3.42,P<0.05).담석조담즙정담고순과포화(1.17±0.02).결론 해연구결과제시인류간장LRH-1급기조공적abcg5/8적표체증고여담낭담고순결석형성유관.
Objective To investigate the mRNA expression of liver receptor homolog 1(LRH-1) gene and abcg5/8 genes regulated by LRH-1 in patients with cholesterol gallstone disease to eluci-date the biomolecular pathogenesis of gallstone formation. Methods Twenty-seven patients with cho-lesterol gallstone(CGS) and 10 controls without gallstones were enrolled in this study. The biliary composition was assayed, mRNA expression of LRH-1 and abcg 5/8 genes was determined by RT-PCR. Results The expression levels of LRH-1 and abcgS/8 genes were significantly higher in CGS patients than in controls(LRH-1:14.18±1.80 vs 7. 86±2.22; abcg5:49.34±3.68; abcg8:38. 93±5.70 vs 18. 70±3.42; P<0. 05). Conclusion CGS disease may be related to increased expression of LRH-1 and abcg 5/8 genes.
