CAJ | 학술논문

目的利用减毒鼠伤寒沙门菌作为载体,探讨丙型肝炎病毒(HCV)口服DNA疫苗的可行性.方法把HCV复合多表位抗原基因PCX克隆到真核表达载体pcDNA3(CMV启动子),构建HCV真核表达载体pcDNA3/PCX,转化减毒鼠伤寒沙门菌SL3261,获得HCV重组口服DNA活菌苗SL3261(pcDNA3/PCX),免疫小鼠及家兔后,检测特异性免疫应答及安全性.结果 SL3261(peDNA3/PCX)在小鼠及家兔中均可诱发低水平的特异性体液免疫及细胞免疫应答,免疫动物未见明显的毒性反应.结论 HCV口服减毒鼠伤寒沙门菌DNA疫苗可诱发特异性的免疫应答,为HCV疫苗的研究提供新的理论及实验依据.
목적 이용감독서상한사문균작위재체,탐토병형간염병독(HCV)구복DNA역묘적가행성.방법 파HCV복합다표위항원기인PCX극륭도진핵표체재체pcDNA3(CMV계동자),구건HCV진핵표체재체pcDNA3/PCX,전화감독서상한사문균SL3261,획득HCV중조구복DNA활균묘SL3261(pcDNA3/PCX),면역소서급가토후,검측특이성면역응답급안전성.결과 SL3261(peDNA3/PCX)재소서급가토중균가유발저수평적특이성체액면역급세포면역응답,면역동물미견명현적독성반응.결론 HCV구복감독서상한사문균DNA역묘가유발특이성적면역응답,위HCV역묘적연구제공신적이론급실험의거.
Objective To use Salmonella typhimurium as a vehicle to explore the possibility of oral live DNA vaccine against hepatitis C virus(HCV).Methods A multi-epitopes antigen gene of HCV was cloned into a eukaryotic vector pcDNA3 and introduced into attenuated Salmonella typhimurium SL3261 to construct HCV oral lire DNA vaccine candidate SL3261(pcDNA3/PCX).The recombinant bacterium was used to orally immunize mice and rabbits.Meanwhile,specific humoral and cellular immune responses and safetv were detected.Results Low level of specific antibodies and cellular immune responses were induced without obyious toxicity in the immunized mice and rabbits after oral administration of the Iive bacteria.Conclusion The results indicate that the HCV recombinant oral live DNA vaccine can induce specific immune responses which might be able to provide an alternative for HCV vaccines.