CAJ | 학술논문

目的评价芬太尼和舒芬太尼对左旋甲状腺素钠预处理大鼠心肌保护作用的影响.方法幼鼠采用随机数字表法随机分为7组(每组8只):空白对照组(BC组)、对照组(C组)、左旋甲状腺素钠10 μg组(10 μg组)、芬太尼组(F组)、舒芬太尼组(S组)、芬太尼联合左旋甲状腺素钠组(F+L组)和舒芬太尼联合左旋甲状腺素钠组(S+L组).采用Langendorff装置建立离体心脏缺血/再灌注损伤(ischemia/reperfusion injury,I/RI)模型,F组和F+L组灌注液中芬太尼浓度为30 μg/L,S组和S+L组灌注液中舒芬太尼浓度为3 μg/L.结果再灌注30 min时血流动力学F组和S组明显差于BC组；10 μg组、F+L组和S+L组优于C组、F组和S组.BC组、C组、10 μg组、F组、S组、F+L组和S+L组再灌注期的冠脉流量分别是(19±2)、(16±1)、(21±2)、(17±1)、(17±1)、(21±1)、(22±1)ml/min,并且10 μg组、F+L组和S+L组的冠脉流量高于C组、F组和S组.冠脉流出液中的肌酸激酶同工酶(creatine kinase,CK-MB)活性C组、F组和S组高于10 μg组、F+L组和S+L组.心肌热休克蛋白70(heatshock proteins70,HSP70)和肌球蛋白重链(myosin heavy-chain,MHC)α mRNA表达10 μg组、F+L组和S+L组明显强于C组、F组和S组.但是所有检测指标在10 μg组、F+L组和S+L组之间差异无统计学意义. 结论芬太尼和舒芬太尼对左旋甲状腺素钠预处理的心肌保护作用均无明显影响.
목적 평개분태니화서분태니대좌선갑상선소납예처리대서심기보호작용적영향.방법 유서채용수궤수자표법수궤분위7조(매조8지):공백대조조(BC조)、대조조(C조)、좌선갑상선소납10 μg조(10 μg조)、분태니조(F조)、서분태니조(S조)、분태니연합좌선갑상선소납조(F+L조)화서분태니연합좌선갑상선소납조(S+L조).채용Langendorff장치건립리체심장결혈/재관주손상(ischemia/reperfusion injury,I/RI)모형,F조화F+L조관주액중분태니농도위30 μg/L,S조화S+L조관주액중서분태니농도위3 μg/L.결과 재관주30 min시혈류동역학F조화S조명현차우BC조；10 μg조、F+L조화S+L조우우C조、F조화S조.BC조、C조、10 μg조、F조、S조、F+L조화S+L조재관주기적관맥류량분별시(19±2)、(16±1)、(21±2)、(17±1)、(17±1)、(21±1)、(22±1)ml/min,병차10 μg조、F+L조화S+L조적관맥류량고우C조、F조화S조.관맥류출액중적기산격매동공매(creatine kinase,CK-MB)활성C조、F조화S조고우10 μg조、F+L조화S+L조.심기열휴극단백70(heatshock proteins70,HSP70)화기구단백중련(myosin heavy-chain,MHC)α mRNA표체10 μg조、F+L조화S+L조명현강우C조、F조화S조.단시소유검측지표재10 μg조、F+L조화S+L조지간차이무통계학의의. 결론분태니화서분태니대좌선갑상선소납예처리적심기보호작용균무명현영향.
Objective To evaluate influences of fentanyl and sufentauil on the cardioprotection effect of preemptive levothyroxine-sodium administration in the rats. Methods Immature rats were randomly allocated into seven groups (n=8):blank control group (group BC),control group (group C),levothyroxine-sodium 10 μg group(group 10 μg),fentanyl group (group F),sufentanil group (group S),combined fentanyl and levothyroxine-sodium group (group F+L) and combined sufentanil and levothyroxine-sodium group(group S+L).The isolated heart model was established with a Langendorff apparatus.Hearts in the groups F and F+L were perfused with KH fluid containing fentanyl 30 μg/L,and hearts in the groups S and S+L were perfused with KH fluid containing sufentanil 3 μg/L. Results The hemodynamic variables at 30 min of the reperfusion were inferior in group F and S than in group BC,but better in group 10 μg,group F+L and group S+L than in group C,F and S.The coronary perfusion flows in group BC,group C,group 10 μg,group F,group S,group F+L and group S+L were (19±2),(16±1),(21±2),(17±1),(17±1),(21±1),(22±1) ml/min,respectively,with a significantly higher coronary perfusion flow in group 10 μg,group F+L and group S+L than in group C,group F and group S.The myocardial bound creatine kinase (CK-MB) level in the coronary perfusion fluid during the reperfusion period was significandy higher in groups C,F and S compared with groups 10 μg,F+L and S+L.The expression of b oth heat shock proteins70(HSP70) and myosin heavy-chain(MHC) mRNA in the myocardium was significantly higher in groups 10 μg,F+L and S+L than in groups C,F and S.However,there were no differences in all the observed parameters anong groups 10 μg,F+L and S +L. Conclusions Both fentanyl and sufentanil do not produce significant influence on the cardioprotective effect of levothyroxine sodium preconditioning.