CAJ | 학술논문

首先采用纤维素酶、果胶酶、木瓜蛋白酶,分别对灰树花子实体进行酶解提取灰树花多糖,经正交试验获得各种酶的最佳工艺参数;然后依次分步采用纤维素酶、果胶酶和木瓜蛋白酶组合处理灰树花子实体提取灰树花多糖,并与传统的热水浸提法进行对比。结果表明：分步酶解法提取灰树花粗多糖的提取率可达28.62%,比传统的热水浸提法提取灰树花粗多糖的提取率提高了40.4%。经紫外光谱和红外图谱分析表明,分步酶解法提取灰树花多糖的纯度明显高于热水浸提法提取的灰树花多糖。
수선채용섬유소매、과효매、목과단백매,분별대회수화자실체진행매해제취회수화다당,경정교시험획득각충매적최가공예삼수;연후의차분보채용섬유소매、과효매화목과단백매조합처리회수화자실체제취회수화다당,병여전통적열수침제법진행대비。결과표명：분보매해법제취회수화조다당적제취솔가체28.62%,비전통적열수침제법제취회수화조다당적제취솔제고료40.4%。경자외광보화홍외도보분석표명,분보매해법제취회수화다당적순도명현고우열수침제법제취적회수화다당。
The Griflan was extracted from fruit bodies of Grifola frondosa by means of adding cellulose,pectase and papain respectively.The best technological factors were obtained for each enzyme from the the orthogonal test.Then the fruit bodies of Grifola frondosa was treated by adding the three enzymes successively under their best conditions.Compared with the result from the hot water extraction technology,the yield 28.62% of Griflan by step enzymic hydrolysis was raised as high as 40.4%.From the ultraviolet spectrum and infrared spectrum,it was found that the purity of Griflan was improved dramatically by the new technology.