中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2011年
11期
35-38
,共4页
猪繁殖与呼吸综合症%NSP2基因%ORF5基因%变异分析
豬繁殖與呼吸綜閤癥%NSP2基因%ORF5基因%變異分析
저번식여호흡종합증%NSP2기인%ORF5기인%변이분석
Porcine reproductive and respiratory syndrome viruses%NSP2 gene%ORF5 gene%variation analysis
为了给广西柳州预防和控制猪繁殖与呼吸综合症提供理论数据,本研究对利用RT-PCR方法扩增了该地区流行株LZ5和LZ6的NSP2和ORF5基因并进行测序分析,然后与GenBank中已发表的PRRSV毒株的NSP2和ORF5基因序列进行比较。结果显示,2株柳州地方流行株均属于美洲型,在NSP2基因第483位和535~563位氨基酸均存在30个氨基酸的不连续缺失,其NSP2和ORF5基因的核苷酸同源性分别为99.5%和99.7%,推导的氨基酸同源性分别为100%和99.5%;与近几年国内流行的高致病性蓝耳病代表性毒株之间的NSP2、ORF5基因核苷酸同源性分别为94.7%~96.2%和97.2%~98.0%,其推导的氨基酸同源性分别为92.0%~95.5%和94.5%~97.0%。序列分析结果表明此次柳州流行的PRRSV与2006年夏高热病引起的基因序列高度同源,在基因序列上并未显示出新的特性,在国内也缺乏明显的地域性差异。
為瞭給廣西柳州預防和控製豬繁殖與呼吸綜閤癥提供理論數據,本研究對利用RT-PCR方法擴增瞭該地區流行株LZ5和LZ6的NSP2和ORF5基因併進行測序分析,然後與GenBank中已髮錶的PRRSV毒株的NSP2和ORF5基因序列進行比較。結果顯示,2株柳州地方流行株均屬于美洲型,在NSP2基因第483位和535~563位氨基痠均存在30箇氨基痠的不連續缺失,其NSP2和ORF5基因的覈苷痠同源性分彆為99.5%和99.7%,推導的氨基痠同源性分彆為100%和99.5%;與近幾年國內流行的高緻病性藍耳病代錶性毒株之間的NSP2、ORF5基因覈苷痠同源性分彆為94.7%~96.2%和97.2%~98.0%,其推導的氨基痠同源性分彆為92.0%~95.5%和94.5%~97.0%。序列分析結果錶明此次柳州流行的PRRSV與2006年夏高熱病引起的基因序列高度同源,在基因序列上併未顯示齣新的特性,在國內也缺乏明顯的地域性差異。
위료급엄서류주예방화공제저번식여호흡종합증제공이론수거,본연구대이용RT-PCR방법확증료해지구류행주LZ5화LZ6적NSP2화ORF5기인병진행측서분석,연후여GenBank중이발표적PRRSV독주적NSP2화ORF5기인서렬진행비교。결과현시,2주류주지방류행주균속우미주형,재NSP2기인제483위화535~563위안기산균존재30개안기산적불련속결실,기NSP2화ORF5기인적핵감산동원성분별위99.5%화99.7%,추도적안기산동원성분별위100%화99.5%;여근궤년국내류행적고치병성람이병대표성독주지간적NSP2、ORF5기인핵감산동원성분별위94.7%~96.2%화97.2%~98.0%,기추도적안기산동원성분별위92.0%~95.5%화94.5%~97.0%。서렬분석결과표명차차류주류행적PRRSV여2006년하고열병인기적기인서렬고도동원,재기인서렬상병미현시출신적특성,재국내야결핍명현적지역성차이。
In order to offer the theoretical data for prevention and control of PRRS endemic in Liuzhou region,the partial NSP2 and ORF5 genes of PRRSV LZ5 and LZ6 strains were amplified by RT-PCR and sequenced.The obtained sequences were analyzed and compared with the published nucleotide sequences of other strains in GeneBank by DNAstar software.The results showed that the two strains were classified into the North American type of PRRSV.The partial NSP2 gene shared a common feature of two distinctive deletions in 483 and 535-563 amino acid, a 30 amino acids deletion in NSP2 were found. Sequence comparison of partial NSP2 and ORF5 gene revealed a sequence homology of 99.5% and 99.7% between them, with a similarity of 100% and 99.5% in deduced amino acid sequences respectively.Their NSP2 and ORF5 gene shared 94.7%-96. 2% and 97.2%-98.0% of nucleotide homology, and 92.0%-95.5% and 94.5%-97.0% of amino acid identity with the very viru- lent PRRSV strains which isolated in our country in recent years respectively.In conclusion, the PRRSV strains prevailed in the field of Liuzhou were highly homologous to the wave of PRRS occurred in 2006,with no new characteristics in gene sequence, and also with no distinct difference in geological origins.
