农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2011年
11期
1640-1644
,共5页
祝朋芳%赵妮拉%齐丹%刘娜%段玉玺
祝朋芳%趙妮拉%齊丹%劉娜%段玉璽
축붕방%조니랍%제단%류나%단옥새
菊花白锈病%堀氏菊柄锈菌%抗性鉴定标准%离体%显微镜检
菊花白鏽病%堀氏菊柄鏽菌%抗性鑒定標準%離體%顯微鏡檢
국화백수병%굴씨국병수균%항성감정표준%리체%현미경검
Chrysanthemum white rust (CWR)%Puccinia horiana Henn%Identification standards%In vitro%Microscopy test
[目的]探讨在可控条件下接种和鉴定菊花白锈病的有效方法。[方法]笔者将目测观察与显微镜检方法相结合,制定了菊花白锈病6级抗性鉴定标准,并优化了人工离体接种方法。[结果]结果表明,培养瓶内茎段扦插法、培养皿内平铺叶片法均可用于菊花白锈病的离体鉴定;培养瓶内茎段扦插法优于培养皿内平铺叶片法,是最佳的人工离体接种鉴定方法。[结论]本研究为安全有效地进行菊花白锈病的相关研究提供科学方法,以便最大程度地控制该病的进一步发生和流行。
[目的]探討在可控條件下接種和鑒定菊花白鏽病的有效方法。[方法]筆者將目測觀察與顯微鏡檢方法相結閤,製定瞭菊花白鏽病6級抗性鑒定標準,併優化瞭人工離體接種方法。[結果]結果錶明,培養瓶內莖段扢插法、培養皿內平鋪葉片法均可用于菊花白鏽病的離體鑒定;培養瓶內莖段扢插法優于培養皿內平鋪葉片法,是最佳的人工離體接種鑒定方法。[結論]本研究為安全有效地進行菊花白鏽病的相關研究提供科學方法,以便最大程度地控製該病的進一步髮生和流行。
[목적]탐토재가공조건하접충화감정국화백수병적유효방법。[방법]필자장목측관찰여현미경검방법상결합,제정료국화백수병6급항성감정표준,병우화료인공리체접충방법。[결과]결과표명,배양병내경단천삽법、배양명내평포협편법균가용우국화백수병적리체감정;배양병내경단천삽법우우배양명내평포협편법,시최가적인공리체접충감정방법。[결론]본연구위안전유효지진행국화백수병적상관연구제공과학방법,이편최대정도지공제해병적진일보발생화류행。
[Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. [Method] By combining the observation methods with the naked eye and under optical microscope, we had established the identification standards for chrysanthemum white rust with six classifications and optimized artificial inoculation methods in vitro. [Result] The results showed that bottled cuttings identification method and petri dished leaves identification method both can be used for identification in vitro of chrysanthemum white rust, bottled cuttings identification method had shown better effects than petri dished leaves identification method, and was supposed to be best artificial inoculation and identification method in vitro. [Conclusion] This research had provided a scientific method for safe and effective researches on chrysanthemum white rust, in order to control the occurrence and diffusion of this quarantine disease.