中国动物传染病学报
中國動物傳染病學報
중국동물전염병학보
CHINESE JOURNAL OF VETERINARY PARASITOLOGY
2011年
4期
48-54
,共7页
李东%朱珠%石耀军%陆柯%彭金彪%洪炀%张旻%韩彦辉
李東%硃珠%石耀軍%陸柯%彭金彪%洪煬%張旻%韓彥輝
리동%주주%석요군%륙가%팽금표%홍양%장민%한언휘
日本血吸虫抱雌沟蛋白%免疫机制%淋巴细胞亚群%细胞因子
日本血吸蟲抱雌溝蛋白%免疫機製%淋巴細胞亞群%細胞因子
일본혈흡충포자구단백%면역궤제%림파세포아군%세포인자
Schistosomajaponicum%gynecophoral canal protein%immune mechanism%lymphocyte subsets%cytokine
应用大肠杆菌表达日本血吸虫抱雌沟蛋白（Schistosoma japonicum gynecophoral canal protein, SjGCP）。将重组蛋白rSjGCP与FCA、ISA206、ISA70M三种佐剂混合后免疫BALB／c小鼠，分析重组抗原诱导的免疫保护机制。用流式细胞术检测三次免疫后小鼠体内CD4＋、CD8＋T淋巴细胞亚群和IL-2、IL-4、γ—IFN、IL-10等细胞因子，应用酶联免疫吸附试验（ELISA）检测抗SjGCP特异性1gG抗体及其亚型lgG1水平。和空白对照组相比，GCP—FCA组和GCP-206组小鼠脾细胞中CD8＋T淋巴细胞比例显著下降，而GCP-70M组的CD4＋T淋巴细胞显著下降；GCP—FCA组、GCP-206组中IL-4、IL-10表达水平均显著上升；GCP—FCA组、GCP-206组、GCP-70M组血清中特异性IgG1亚型抗体含量显著升高。本研究为探讨重组日本血吸虫抱雌沟蛋白诱导的免疫保护机制积累了有价值的数据。
應用大腸桿菌錶達日本血吸蟲抱雌溝蛋白（Schistosoma japonicum gynecophoral canal protein, SjGCP）。將重組蛋白rSjGCP與FCA、ISA206、ISA70M三種佐劑混閤後免疫BALB／c小鼠，分析重組抗原誘導的免疫保護機製。用流式細胞術檢測三次免疫後小鼠體內CD4＋、CD8＋T淋巴細胞亞群和IL-2、IL-4、γ—IFN、IL-10等細胞因子，應用酶聯免疫吸附試驗（ELISA）檢測抗SjGCP特異性1gG抗體及其亞型lgG1水平。和空白對照組相比，GCP—FCA組和GCP-206組小鼠脾細胞中CD8＋T淋巴細胞比例顯著下降，而GCP-70M組的CD4＋T淋巴細胞顯著下降；GCP—FCA組、GCP-206組中IL-4、IL-10錶達水平均顯著上升；GCP—FCA組、GCP-206組、GCP-70M組血清中特異性IgG1亞型抗體含量顯著升高。本研究為探討重組日本血吸蟲抱雌溝蛋白誘導的免疫保護機製積纍瞭有價值的數據。
응용대장간균표체일본혈흡충포자구단백（Schistosoma japonicum gynecophoral canal protein, SjGCP）。장중조단백rSjGCP여FCA、ISA206、ISA70M삼충좌제혼합후면역BALB／c소서，분석중조항원유도적면역보호궤제。용류식세포술검측삼차면역후소서체내CD4＋、CD8＋T림파세포아군화IL-2、IL-4、γ—IFN、IL-10등세포인자，응용매련면역흡부시험（ELISA）검측항SjGCP특이성1gG항체급기아형lgG1수평。화공백대조조상비，GCP—FCA조화GCP-206조소서비세포중CD8＋T림파세포비례현저하강，이GCP-70M조적CD4＋T림파세포현저하강；GCP—FCA조、GCP-206조중IL-4、IL-10표체수평균현저상승；GCP—FCA조、GCP-206조、GCP-70M조혈청중특이성IgG1아형항체함량현저승고。본연구위탐토중조일본혈흡충포자구단백유도적면역보호궤제적루료유개치적수거。
For investigating the immune mechanism induced by the vaccine candidate Schistosoma japonicum gynecophoral canal protein（SjGCP）, BALB/c mice were vaccinated with the recombinant protein SjGCP mixed with three different adjuvants FCA, ISA 206 and ISA 70M, respectively. The percentage of CD4＋ and CD8＋ T lymphocyte subsets and the cytokines IL-2, IL-4, γ-IFN and IL-10 in splenic lymphocytes from mice post three immunizations were analyzed by flow cytometry, and the serum of each groups were collected for detecting specific antibodies IgG and IgG1 by ELISA. The results showed that the percentage of CD4＋ T cells in GCP-70M group and CDS＋ T cells in GCP-FCA and GCP-206 groups decreased significantly post three immunizations, comparing to the PBS control group, while the percent of CD3＋CD8-IL-4＋ and CD3＋CD8-IL-10＋ cells in groups of GCP-FCA and GCP-206 increased. The ELISA results showed that the levels of the specific antibodies IgG 1 subtype in the sera of GCP-FCA, GCP-206 and GCP-70M groups were significantly higher than that of control groups. These results accumulated the valuable data for understanding the protection mechanism induced by recombinant protein SjGCP.
