延边大学农学学报
延邊大學農學學報
연변대학농학학보
JOURNAL OF AGRICULTURAL SCIENCE YANBIAN UNIVERSITY
2011年
3期
168-171,188
,共5页
苏云金杆菌%CAB109菌株%杀虫晶体蛋白%防效
囌雲金桿菌%CAB109菌株%殺蟲晶體蛋白%防效
소운금간균%CAB109균주%살충정체단백%방효
Bacillus thuringiensis%CAB109%insecticidal crystal proteins%control effect
对甜菜夜蛾等害虫具有高毒力的苏云金杆菌CAB109菌株进行生物学特性调查结果表明，CAB109菌株的伴孢晶体具有典型的菱形结构；其原毒素蛋白的分子量为133kDa，经胰蛋白酶处理后水解为66kDa的活性毒素蛋白．DNA凝胶电泳显示：CAB19菌株的质粒DNA与同样H，血清型的已知HD-133菌株不同．CAB109菌株对葱田甜菜夜蛾的平均防效达75．3％，高于常用化学农药和微生物农药的防效；平均挽回损失率也达26．0%表现出优良的开发潜力．
對甜菜夜蛾等害蟲具有高毒力的囌雲金桿菌CAB109菌株進行生物學特性調查結果錶明，CAB109菌株的伴孢晶體具有典型的蔆形結構；其原毒素蛋白的分子量為133kDa，經胰蛋白酶處理後水解為66kDa的活性毒素蛋白．DNA凝膠電泳顯示：CAB19菌株的質粒DNA與同樣H，血清型的已知HD-133菌株不同．CAB109菌株對蔥田甜菜夜蛾的平均防效達75．3％，高于常用化學農藥和微生物農藥的防效；平均輓迴損失率也達26．0%錶現齣優良的開髮潛力．
대첨채야아등해충구유고독력적소운금간균CAB109균주진행생물학특성조사결과표명，CAB109균주적반포정체구유전형적릉형결구；기원독소단백적분자량위133kDa，경이단백매처리후수해위66kDa적활성독소단백．DNA응효전영현시：CAB19균주적질립DNA여동양H，혈청형적이지HD-133균주불동．CAB109균주대총전첨채야아적평균방효체75．3％，고우상용화학농약화미생물농약적방효；평균만회손실솔야체26．0%표현출우량적개발잠력．
Bacillus thuringiensis isolate CAB109 was bipyramidal shape by microscopic examination which had high insecticidal activity to Spodoptera exigua. The molecular weight of this isolate was 133 kDa, and the molecular weight of active protein toxin was 66 kDa after treated with trypsin. DNA analysis showed that plasmid DNA of CAB109 was different from B. thuringiensis subsp, aizawai HD--133 which has same H7 serotype. Mortality and damaged reduce of S. exigua treated by CAB109 could reached 75.3% and 26.0%,respectively. The effect were better than ordinary chemical and microbe pesticides treatments. This study showed excellent development potential of B. thuringiensis isolate CAB109.
