CAJ | 학술논문

对从病死锦鲤（cyprinuscarpioL．）肝组织中分离的2株菌（编号：HC050630B-1，HC050630B-2）进行了形态特征、主要理化特性、对健康鲤鱼的致病作用、药物敏感性等方面的检验；同时测定了HCOS0630B-1株菌的16SrRNA基因序列，构建了系统发育树。结果表明，2株被检菌为弗氏柠檬酸杆菌（Citrobacter加吼蒯），对健康鲤鱼有致病作用；所测菌株的16SrRNA基因序列长度1458bp，在GenBank中登录号为EF669481，该菌株16SrRNA基因序列与GenBank数据库中柠檬酸杆菌属细菌的16SrRNA基因序列同源性在99％；药敏试验结果显示，对供试的头孢噻肟等21种药物呈现高度敏感或敏感，对头孢唑啉等5种呈低度敏感，对四环素等11种耐药。
대종병사금리（cyprinuscarpioL．）간조직중분리적2주균（편호：HC050630B-1，HC050630B-2）진행료형태특정、주요이화특성、대건강리어적치병작용、약물민감성등방면적검험；동시측정료HCOS0630B-1주균적16SrRNA기인서렬，구건료계통발육수。결과표명，2주피검균위불씨저몽산간균（Citrobacter가후괴），대건강리어유치병작용；소측균주적16SrRNA기인서렬장도1458bp，재GenBank중등록호위EF669481，해균주16SrRNA기인서렬여GenBank수거고중저몽산간균속세균적16SrRNA기인서렬동원성재99％；약민시험결과현시，대공시적두포새우등21충약물정현고도민감혹민감，대두포서람등5충정저도민감，대사배소등11충내약。
Two strains （ HC050630B-1 ,HC050630B-2） isolated from the liver of diseased （ or dead） ornamen- tal carp were examined,including their morphological characteristics,physiological and biochemical character- istics ,pathogenicity to healthy carp and antibiotic sensitivity. In addition,the 16S rRNA gene of HC050630B-1 were sequenced,and molecular phylogenetic tree was constructed. The results showed that the two examined strains were identified as Citrobacter freundii, and had strong pathogenicity to healthy carp. The length of the 16S rRNA gene sequence of examined strain is 1 458 bp. Its database accession numbers in NCBI is EF669481 ,and the similarity of the 16S rRNA gene sequence of examined strain with the 16S rRNA gene of Citrobacter from GenBank database is 99%. The antibiotic sensitivity using 37 antimicrobial agents showed that isolates were high sensitive or sensitive to 21 agents including cefotaxime, slight sensitive to 5 agents including cefazolin, and resistant to 11 agents including tetrecycline.