CAJ | 학술논문

目的：建立稳定遗传的纯合子SV40T胃壁细胞定位表达转基因小鼠品系。方法：通过定量PCR法比较已知杂合子小鼠和未知阳性小鼠中外源基因的起始模板量来确定小鼠的基因型,并通过测交的方法来验证试验结果。结果：用定量PCR法选育出15只纯合子小鼠,经测交验证它们与野生型小鼠交配所生的后代均为阳性,证明了定量PCR的结果是正确的。通过纯合子之间的全同胞交配建立了6个独立的纯合子品系。结论：定量PCR具有省时、省力及高通量等优点,能够很好地应用于筛选纯合子转基因动物。
목적：건립은정유전적순합자SV40T위벽세포정위표체전기인소서품계。방법：통과정량PCR법비교이지잡합자소서화미지양성소서중외원기인적기시모판량래학정소서적기인형,병통과측교적방법래험증시험결과。결과：용정량PCR법선육출15지순합자소서,경측교험증타문여야생형소서교배소생적후대균위양성,증명료정량PCR적결과시정학적。통과순합자지간적전동포교배건립료6개독립적순합자품계。결론：정량PCR구유성시、성력급고통량등우점,능구흔호지응용우사선순합자전기인동물。
Aim：To establish homozygote mouse strains which express SV40T in parietal cells.Methods：The original template capacity between the known heterozygote mice and the unknown mice were compared by using real-time PCR to ensure their genotype, and then the results were validated by using cross test.Results： A total of 15 homozygous mice were selected by real-time PCR. The offspring of the homozygote mice and wild mice were all positive, the results of cross test were consistent with the real-time PCR.A total of 6 homozygote mouse strains had been established by mating among the homozygous compatriots.Conclusion： The real-time PCR is a speedy,convenient method with high-flux,hence could be used to estimate the genotype of transgenic mice.