食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
7期
199-204
,共6页
雷福厚%卢建芳%赵慷%覃冬梅%姚兴东
雷福厚%盧建芳%趙慷%覃鼕梅%姚興東
뢰복후%로건방%조강%담동매%요흥동
松香基功能高分子%固定化酶%淀粉酶%稀土离子
鬆香基功能高分子%固定化酶%澱粉酶%稀土離子
송향기공능고분자%고정화매%정분매%희토리자
rosin based functional polymers%enzyme immobilization%amylase%rare earth ion
分别以聚马来松香己二醇酯、马来松香乙二醇丙烯酯聚合物和马来松香乙二醇丙烯酯-丙烯酸共聚物为载体,稀土离子为桥键配离子固定化淀粉酶.测定固定化淀粉酶的性能,探讨固定化酶反应机理.结果表明,稀土离子作为桥键配离子的固定化淀粉酶中,PMGAE Y(Ⅲ)En和PMGAEDy(Ⅲ)En效果较好;最适宜温度均为60℃,最适宜pH值均为6.03:重复使用4次后,活性分别为31.49、29.76mg/g·5min.此外,建立了固定化酶活性与功能高分子微孔面积及酸值间的数学关系式.
分彆以聚馬來鬆香己二醇酯、馬來鬆香乙二醇丙烯酯聚閤物和馬來鬆香乙二醇丙烯酯-丙烯痠共聚物為載體,稀土離子為橋鍵配離子固定化澱粉酶.測定固定化澱粉酶的性能,探討固定化酶反應機理.結果錶明,稀土離子作為橋鍵配離子的固定化澱粉酶中,PMGAE Y(Ⅲ)En和PMGAEDy(Ⅲ)En效果較好;最適宜溫度均為60℃,最適宜pH值均為6.03:重複使用4次後,活性分彆為31.49、29.76mg/g·5min.此外,建立瞭固定化酶活性與功能高分子微孔麵積及痠值間的數學關繫式.
분별이취마래송향기이순지、마래송향을이순병희지취합물화마래송향을이순병희지-병희산공취물위재체,희토리자위교건배리자고정화정분매.측정고정화정분매적성능,탐토고정화매반응궤리.결과표명,희토리자작위교건배리자적고정화정분매중,PMGAE Y(Ⅲ)En화PMGAEDy(Ⅲ)En효과교호;최괄의온도균위60℃,최괄의pH치균위6.03:중복사용4차후,활성분별위31.49、29.76mg/g·5min.차외,건립료고정화매활성여공능고분자미공면적급산치간적수학관계식.
With polyhexmethylene maleic rosinate,polyethylene maleic rosinate polymer or its copolymer with acrylic acid as matrix,amylase was immobilized on La(Ⅲ),Eu(Ⅲ),Tb(Ⅲ),Y(Ⅲ)or Dy(Ⅲ)coordinates of the matrix.The performance of the immobilized enzyme and the mechanism of immobilization were investigated.High activities were observed for the Y(Ⅲ)and Dy(Ⅲ)coordinates of the functional polymers.The optimal temperature and pH were both 60℃ and 6.03,respectively.activities of the immobilized enzymes and surface area of the matrix as well as pH was established.
