CAJ | 학술논문

采用水、95%乙醇及其分别与超声波的综合作用从原蜂胶中提取蜂胶液,将得到的蜂胶提取液稀释成8、4、2、1 mL/L,并分别对蜡样芽胞杆菌、大肠杆菌、金黄色葡萄球菌、沙门氏菌进行抑菌圈直径和最小抑制浓度(M/C)的测定,选取提取效果最好方法中的两个浓度进行鸡蛋保鲜效果试验.结果表明,超声波作用于95%的乙醇蜂胶液的方法提取效果最好;不同提取方法得到的蜂胶提取液的抑菌作用存在差异,以超声波和乙醇提取的蜂胶液对受试菌作用最强;蜂胶提取液对大肠杆菌的抑制作用最强,其次是沙门氏菌和蜡样芽胞杆菌;在鲜蛋保鲜试验中发现,两种浓度、两种方法均有不同程度的保鲜效果.
채용수、95%을순급기분별여초성파적종합작용종원봉효중제취봉효액,장득도적봉효제취액희석성8、4、2、1 mL/L,병분별대사양아포간균、대장간균、금황색포도구균、사문씨균진행억균권직경화최소억제농도(M/C)적측정,선취제취효과최호방법중적량개농도진행계단보선효과시험.결과표명,초성파작용우95%적을순봉효액적방법제취효과최호;불동제취방법득도적봉효제취액적억균작용존재차이,이초성파화을순제취적봉효액대수시균작용최강;봉효제취액대대장간균적억제작용최강,기차시사문씨균화사양아포간균;재선단보선시험중발현,량충농도、량충방법균유불동정도적보선효과.
The propolis extracted by the four methods was diluted into 8,4,2,1mL/L,and its antibacterial activities against Bacillus cereus,Escherichia coil,Staphylococcus aureus,Salmonella sp were investigated by measuring diameters of inhibiting zones and MIC.The results showed that the extraction rate of 95% ethanol and ultrasonic wave was higher;the bacteria could be inhibited by the liquid of propolis in varying degrees.The antibacterial activity of propolis extracted by 95% ethanol and ultrasonic wave was stronger.It was also found that the inhibition effect of propolis on Escherichia coli was the best.