CAJ | 학술논문

目的从定性定量角度探讨粉防己碱诱导宫颈癌HeLa细胞凋亡的作用.方法采用MTT法检测不同浓度粉防己碱作用不同时间对人宫颈癌HeLa细胞株的增殖抑制作用.通过流式细胞仪和激光共聚焦显微镜检测细胞凋亡情况.结果粉防己碱对宫颈癌HeLa细胞具有增殖抑制作用,且呈时间、浓度依赖性.应用流式细胞仪检测到15μmol/L粉防己碱诱导HeLa细胞产生的凋亡率为(51.8±0.97)%,显著高于对照组凋亡率(24.3±1.23)%(P<0.05).通过激光共聚焦显微镜观察,与对照组比较,粉防己碱作用后HeLa细胞具有明显的凋亡形态.结论粉防己碱能够抑制HeLa细胞的增殖并诱导其凋亡.
목적 종정성정량각도탐토분방기감유도궁경암HeLa세포조망적작용.방법 채용MTT법검측불동농도분방기감작용불동시간대인궁경암HeLa세포주적증식억제작용.통과류식세포의화격광공취초현미경검측세포조망정황.결과 분방기감대궁경암HeLa세포구유증식억제작용,차정시간、농도의뢰성.응용류식세포의검측도15μmol/L분방기감유도HeLa세포산생적조망솔위(51.8±0.97)%,현저고우대조조조망솔(24.3±1.23)%(P<0.05).통과격광공취초현미경관찰,여대조조비교,분방기감작용후HeLa세포구유명현적조망형태.결론 분방기감능구억제HeLa세포적증식병유도기조망.
Objective To study the effects of tetrandrine on apoptosis of HeLa cervical cancer cells qualitatively and quantitatively. Methods We measured tetrandrine-induced inhibition of HeLa cell proliferation at different concentrations and time points by MTT assay. The rate of Hela cell apoptosis induced by tetrandrine was detected by flow cytometer and confocal laser scanning microscope (CLSM). Results Tetrandrine inhibited the proliferation of HeLa cells in dosage- and time-dependent manners. Flow cytometry showed that the apoptosis rate was (51.8±0.97)% at the concentration of 15μmol/L, which was significantly higher than that in the control group (24.3±1.23)% (P<0.05). The cells treated with tetrandrine showed typical apoptotic morphology under CLSM. Conclusion Tetrandrine can inhibit proliferation and induce apoptosis of HeLa cervical cancer cells.