浙江大学学报(农业与生命科学版)
浙江大學學報(農業與生命科學版)
절강대학학보(농업여생명과학판)
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2009年
4期
425-432
,共8页
王宏宝%戚龙君%王金成%宋绍祎%林茂松
王宏寶%慼龍君%王金成%宋紹祎%林茂鬆
왕굉보%척룡군%왕금성%송소의%림무송
甘薯%马铃薯腐烂线虫%致病力%繁殖力%性比
甘藷%馬鈴藷腐爛線蟲%緻病力%繁殖力%性比
감서%마령서부란선충%치병력%번식력%성비
sweet potato%Ditylenchus destructor%pathogenicity%fecundity%sex ratio
对国内不同地理来源的10个马铃薯腐烂线虫群体进行同工酶表型研究,同时在2个中抗甘薯茎线虫病品种(苏薯9号、宁薯9-9)上测定马铃薯腐烂线虫群体致病力、繁殖力、雌雄比(性比)、甘薯薯块鲜重损失量.结果表明:①10个群体苹果酸脱氢酶(MDH)酶谱分为2个酶型, 酶型Ⅰ的相对迁移率(Rf值)约为0.21、0.26;酶型Ⅱ的Rf值约为0.21、0.26、0.31,而酯酶染色不成形;②在P=0.05水平上,各群体间的致病力、繁殖力、性比差异显著,其中致病力最高的群体来自河北廊坊DeHB,对苏薯9号的致病力为75%;③在苏薯9号和宁薯9-9上,各线虫群体致病力与线虫繁殖力呈正相关;④偏相关分析显示,在苏薯9号上不同线虫群体繁殖力与性比呈负相关,相关系数为-0.213,而在宁薯9-9上不同群体繁殖力与性比呈显著正相关,相关系数为0.901,说明寄主品种的改变对线虫群体性别分化影响显著;⑤在抗甘薯茎线虫病和保鲜力方面,宁薯9-9分别比苏薯9号高33.48%和19.86%.
對國內不同地理來源的10箇馬鈴藷腐爛線蟲群體進行同工酶錶型研究,同時在2箇中抗甘藷莖線蟲病品種(囌藷9號、寧藷9-9)上測定馬鈴藷腐爛線蟲群體緻病力、繁殖力、雌雄比(性比)、甘藷藷塊鮮重損失量.結果錶明:①10箇群體蘋果痠脫氫酶(MDH)酶譜分為2箇酶型, 酶型Ⅰ的相對遷移率(Rf值)約為0.21、0.26;酶型Ⅱ的Rf值約為0.21、0.26、0.31,而酯酶染色不成形;②在P=0.05水平上,各群體間的緻病力、繁殖力、性比差異顯著,其中緻病力最高的群體來自河北廊坊DeHB,對囌藷9號的緻病力為75%;③在囌藷9號和寧藷9-9上,各線蟲群體緻病力與線蟲繁殖力呈正相關;④偏相關分析顯示,在囌藷9號上不同線蟲群體繁殖力與性比呈負相關,相關繫數為-0.213,而在寧藷9-9上不同群體繁殖力與性比呈顯著正相關,相關繫數為0.901,說明寄主品種的改變對線蟲群體性彆分化影響顯著;⑤在抗甘藷莖線蟲病和保鮮力方麵,寧藷9-9分彆比囌藷9號高33.48%和19.86%.
대국내불동지리래원적10개마령서부란선충군체진행동공매표형연구,동시재2개중항감서경선충병품충(소서9호、저서9-9)상측정마령서부란선충군체치병력、번식력、자웅비(성비)、감서서괴선중손실량.결과표명:①10개군체평과산탈경매(MDH)매보분위2개매형, 매형Ⅰ적상대천이솔(Rf치)약위0.21、0.26;매형Ⅱ적Rf치약위0.21、0.26、0.31,이지매염색불성형;②재P=0.05수평상,각군체간적치병력、번식력、성비차이현저,기중치병력최고적군체래자하북랑방DeHB,대소서9호적치병력위75%;③재소서9호화저서9-9상,각선충군체치병력여선충번식력정정상관;④편상관분석현시,재소서9호상불동선충군체번식력여성비정부상관,상관계수위-0.213,이재저서9-9상불동군체번식력여성비정현저정상관,상관계수위0.901,설명기주품충적개변대선충군체성별분화영향현저;⑤재항감서경선충병화보선력방면,저서9-9분별비소서9호고33.48%화19.86%.
Two isozymes from 10 populations of sweet potato stem nematode, Ditylenchus destructor, were analyzed, as well as the weight loss of sweet potato, pathogenicity, fecundity and sex ratio of these 10 populations by inoculation test in two varieties of sweet potato. The results show that among the 10 populations, there were three main bands of malate dehydrogenase (MDH), which were corresponding to two different phenotypes. Rf was about 0.21, 0.26 and the other was 0.21, 0.26 and 0.31, but esterase (EST) was no band. The pathogenicity, fecundity and sex ratio of the 10 populations were significant at the P=0.05 level, the highest pathogenicity population was from DeHB. There were positive relationships between fecundity and pathogenicity on the Sushu No.9 and Ningshu 9-9. Partial correlation analysis showed that there was negative relationship between fecundity and sex ratio of the 10 populations on the Sushu No.9 and significant positive relationship on the Ningshu 9-9, and their coefficients were -0.213, 0.901, respectively, indicating that the sex differentiation of D. destructor was significantly affected by the different varieties of sweet potato. In addition, the Ningshu 9-9 was 33.48% higher than the Sushu No.9 in the resistant to D. destructor, and 19.86% higher in preservation.