CAJ | 학술논문

对3种结构相近的席夫碱四氮大环草酰胺铜配合物(CuL1-3)的化学核酸酶活性进行比较研究.结果表明:这类配合物的化学核酸酶活性与中心金属离子的类型、配体的结构、溶液的pH值、离子强度及配合物的浓度等都有关系.3种配合物表现出来的化学核酸酶活性顺序为CuL3>CuL2>CuL1.CuL3的DNA切割反应表现为典型的假一级连续反应.在80 umol·L-1 CuL3和2mmol·L-1H2O:的存在下,就超螺旋DNA向切口开环型DNA进而向线型DNA的转化而言,反应速率常数分别为0.0440±0.001 5 min-1(K1)和0.003 52±0.000 18 min-1(K2).
대3충결구상근적석부감사담대배초선알동배합물(CuL1-3)적화학핵산매활성진행비교연구.결과표명:저류배합물적화학핵산매활성여중심금속리자적류형、배체적결구、용액적pH치、리자강도급배합물적농도등도유관계.3충배합물표현출래적화학핵산매활성순서위CuL3>CuL2>CuL1.CuL3적DNA절할반응표현위전형적가일급련속반응.재80 umol·L-1 CuL3화2mmol·L-1H2O:적존재하,취초라선DNA향절구개배형DNA진이향선형DNA적전화이언,반응속솔상수분별위0.0440±0.001 5 min-1(K1)화0.003 52±0.000 18 min-1(K2).
The chemical nuclease activities of three closely related Schiff base tetraazamacrocyclic oxamido eopper(Ⅱ) complexes (CuL1-3)were compared and the result shows that their chemical nuclease activities follow the order of CuL3>CuL2>CuL1. Where complexes CuL1-3 are [5,6-d] [13,14-d]dibenzo- 1,4,8,11 -tetraazacyclotetradecanedione-2,3-diene-7,11-di-ethylcarboxylate-7,12 Copper(Ⅱ), [5,6-d][13,14-d]dibenzo-10-methyl-1,4,8,11-tetraazacyclotetradecane-dione-2,3-diene-7,11-di-ethylcarboxylate-7,12 Copper (Ⅱ) and [5,6-d] [13,14-d]dibenzo-[9,10-d]cyclehexano-1,4,8,11-tetraazacyclotetradecane-dione-2,3-diene-7,11-di-ethylcarboxylate-7,12 Copper(Ⅱ), respectively. The interactions of CuL3 with DNA were studied in detail, and the results show that the chemical nuclease activity of this complex is affected by central metal ions, structure of ligands, pH value, ion strength and complex concentration. DNA cleavage reaction in presence of the copper (Ⅱ) complexes is a typical pesudo-first-order consecutive reaction, and the rate constants of 0.044 0±0.001 5 min-1(k1) and 0.003 52±0.000 18 min-1(k2) for the conversion of supercoiled to nicked DNA and nicked to linear DNA are obtained in presence of 80 umol·L-1CuL3 and 2 mmol·L-1H2O2, respectively.