中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2012年
3期
200-204
,共5页
张悦%施晓雷%韩冰%顾劲扬%褚薛慧%肖江强%任昊桢%檀家俊%丁义涛
張悅%施曉雷%韓冰%顧勁颺%褚薛慧%肖江彊%任昊楨%檀傢俊%丁義濤
장열%시효뢰%한빙%고경양%저설혜%초강강%임호정%단가준%정의도
生物人工肝%膜截流分子量%猪肝细胞%骨髓间充质干细胞
生物人工肝%膜截流分子量%豬肝細胞%骨髓間充質榦細胞
생물인공간%막절류분자량%저간세포%골수간충질간세포
Bioartificial liver%Membrane molecular weight cut off%Porcine hepatocyte%Bone marrow mesenchymal stem cells
目的 探讨膜截流分子量对新型生物人工肝(bioartificial liver,BAL)支持系统免疫安全性的影响.方法 应用D氨基半乳糖静脉滴注比格犬建立肝功能衰竭模型.急性肝功能衰竭犬根据BAL隔离膜截流分子量的大小分为两组:A组:200 kD组；B组:1200 kD组.两组均接受新型2次BAL治疗,每次6h.观察各组体内IgG、IgM、50％补体溶血单位(CH50)变化及反应器内抗体漏过情况.结果 在第一次BAL治疗后两组的IgG和IgM水平均没有发生明显的变化.在第2次治疗后第7天,1200 kD组的IgG和IgM水平明显升高,200 kD组IgG和IgM水平仍未出现明显上升或下降.在第一次BAL治疗后,两组CH50都出现暂时性的下降,在治疗后1h达到最低值,而后缓步上升,在7d后恢复至治疗前水平.反应器内培养液中IgG、IgM及CH50检测结果显示,1200 kD组在治疗结束后IgG、IgM及CH50含量显著高于200 kD组.结论 膜截流分子量可能是影响BAL异种免疫排斥的重要因素之一.
目的 探討膜截流分子量對新型生物人工肝(bioartificial liver,BAL)支持繫統免疫安全性的影響.方法 應用D氨基半乳糖靜脈滴註比格犬建立肝功能衰竭模型.急性肝功能衰竭犬根據BAL隔離膜截流分子量的大小分為兩組:A組:200 kD組；B組:1200 kD組.兩組均接受新型2次BAL治療,每次6h.觀察各組體內IgG、IgM、50％補體溶血單位(CH50)變化及反應器內抗體漏過情況.結果 在第一次BAL治療後兩組的IgG和IgM水平均沒有髮生明顯的變化.在第2次治療後第7天,1200 kD組的IgG和IgM水平明顯升高,200 kD組IgG和IgM水平仍未齣現明顯上升或下降.在第一次BAL治療後,兩組CH50都齣現暫時性的下降,在治療後1h達到最低值,而後緩步上升,在7d後恢複至治療前水平.反應器內培養液中IgG、IgM及CH50檢測結果顯示,1200 kD組在治療結束後IgG、IgM及CH50含量顯著高于200 kD組.結論 膜截流分子量可能是影響BAL異種免疫排斥的重要因素之一.
목적 탐토막절류분자량대신형생물인공간(bioartificial liver,BAL)지지계통면역안전성적영향.방법 응용D안기반유당정맥적주비격견건립간공능쇠갈모형.급성간공능쇠갈견근거BAL격리막절류분자량적대소분위량조:A조:200 kD조；B조:1200 kD조.량조균접수신형2차BAL치료,매차6h.관찰각조체내IgG、IgM、50％보체용혈단위(CH50)변화급반응기내항체루과정황.결과 재제일차BAL치료후량조적IgG화IgM수평균몰유발생명현적변화.재제2차치료후제7천,1200 kD조적IgG화IgM수평명현승고,200 kD조IgG화IgM수평잉미출현명현상승혹하강.재제일차BAL치료후,량조CH50도출현잠시성적하강,재치료후1h체도최저치,이후완보상승,재7d후회복지치료전수평.반응기내배양액중IgG、IgM급CH50검측결과현시,1200 kD조재치료결속후IgG、IgM급CH50함량현저고우200 kD조.결론 막절류분자량가능시영향BAL이충면역배척적중요인소지일.
Objective To investigate the influence of membrane molecular weight cut off in our bioartificial liver(BAL)system.Methods Beagle dogs were used for a model of acute liver failure through D-galactosamine administration.The acute liver failure Beagles were divided into two groups by the membrane molecular weight cut off.Group A was treated with BAL containing 200 kDa retention rating membrane.Group B was treated with BAL containing 1200 kDa retention rating membrane.Each group underwent two six-hour BAL treatments that were performed on day 1 and day 21.BAL medium were examined and levels of IgG,IgM,and complement hemolytic unit of 50％(CH50)antibodies were measured in all Beagles and.Results BAL treatment was associated with a significant decline in levels of CH50.1200 kDa group experienced a significant increase in levels of IgG and IgM after two BAL treatments.Significant levels of canine proteins were detected in BAL medium from 1200 kDa group.Conclusions Xenogeneic immune response in the BAL system was influenced by membrane molecular weight cut off.
